Rat Epoxide Hydrolase 1, Microsomal (EPHX1) RTU ELISA Kit | EPHX1 rtu elisa kit

Rat Epoxide Hydrolase 1, Microsomal (EPHX1) ELISA Kit

Cat. Num. AAA459078

• Gene Names: Ephx1; mEH; MEH8
• Reactivity: Rat
• Synonyms: Epoxide Hydrolase 1; Microsomal (EPHX1); Rat Epoxide Hydrolase 1; Microsomal (EPHX1) ELISA Kit; Epoxide hydratase; Microsomal epoxide hydrolase; Eph-1; MEH8; EPHX1 rtu elisa kit

• Reactivity: Rat
• Sequence Length: 455

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates or Other Biological Fluids.
• Detection Range: 31.2-2000pg/mL
• Sensitivity: 13.3pg/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for EPHX1 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to EPHX1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to EPHX1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain EPHX1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of EPHX1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 77539442
• NCBI GeneID: 25315
• NCBI Accession #: NP_001029262.1
• NCBI GenBank Nucleotide #: NM_001034090.3
• UniProt Accession #: P07687
• Molecular Weight: 52,582 Da
• NCBI Official Full Name: epoxide hydrolase 1
• NCBI Official Synonym Full Names: epoxide hydrolase 1
• NCBI Official Symbol: Ephx1
• NCBI Official Synonym Symbols: mEH; MEH8
• NCBI Protein Information: epoxide hydrolase 1
• UniProt Protein Name: Epoxide hydrolase 1
• UniProt Gene Name: Ephx1
• UniProt Synonym Gene Names: Eph-1; mEHCurated

NCBI Description

catalyzes the hydration of a wide range of xenobiotic arene oxides [RGD, Feb 2006]

Uniprot Description

Biotransformation enzyme that catalyzes the hydrolysis of arene and aliphatic epoxides to less reactive and more water soluble dihydrodiols by the trans addition of water. May play a role in the metabolism of endogenous lipids such as epoxide-containing fatty acids.