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Western Blot (WB) (Western blot analysis of KRT4 antibody (C-term) in mouse spleen tissue lysates (35ug/lane). KRT4 (arrow) was detected using the purified Pab.)

Rabbit anti-Human, Mouse KRT4 Polyclonal Antibody | anti-KRT4 antibody

KRT4, CT (Keratin, Type II Cytoskeletal 4, Cytokeratin-4, CK-4, Keratin-4, K4, CYK4, Type-II Keratin Kb4) (MaxLight 550)

Gene Names
KRT4; K4; CK4; CK-4; CYK4; WSN1
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Ammonium Sulfate Precipitation.
Synonyms
KRT4; Polyclonal Antibody; CT (Keratin; Type II Cytoskeletal 4; Cytokeratin-4; CK-4; Keratin-4; K4; CYK4; Type-II Keratin Kb4) (MaxLight 550); anti-KRT4 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human KRT4. Species Crossreactivity: mouse.
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.
Applicable Applications for anti-KRT4 antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes
IHC: 1:50-1:100
FLISA: 1:1,000
WB: 1:50-1:100
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the C-terminal region of human KRT4 (KLH).
Conjugate
MaxLight550
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of KRT4 antibody (C-term) in mouse spleen tissue lysates (35ug/lane). KRT4 (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of KRT4 antibody (C-term) in mouse spleen tissue lysates (35ug/lane). KRT4 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with KRT4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with KRT4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of MDA-231 cells using KRT4 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (Flow cytometric analysis of MDA-231 cells using KRT4 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-KRT4 antibody
References
1. Zhang, J.M., Oral Dis 15(1), 100-105 (2009). 2. Samen, U., Infect. Immun. 75(11), 5405-5414 (2007).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
57,285 Da
NCBI Official Full Name
keratin, type II cytoskeletal 4
NCBI Official Synonym Full Names
keratin 4, type II
NCBI Official Symbol
KRT4
NCBI Official Synonym Symbols
K4; CK4; CK-4; CYK4; WSN1
NCBI Protein Information
keratin, type II cytoskeletal 4; cytokeratin 4; type-II keratin Kb4
UniProt Protein Name
Keratin, type II cytoskeletal 4
Protein Family
UniProt Gene Name
KRT4
UniProt Synonym Gene Names
CYK4; CK-4; K4
UniProt Entry Name
K2C4_HUMAN

Uniprot Description

K4: Heterotetramer of two type I and two type II keratins. Keratin-4 is generally associated with keratin-13. Detected in the suprabasal layer of the stratified epithelium of the esophagus, exocervix, vagina, mouth and lingual mucosa, and in cells and cell clusters in the mucosa and serous gland ducts of the esophageal submucosa. Expressed widely in the exocervix and esophageal epithelium, with lowest levels detected in the basal cell layer. Belongs to the intermediate filament family.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: intermediate filament cytoskeleton; cell surface; keratin filament; intermediate filament; nucleus

Molecular Function: protein binding; structural molecule activity

Biological Process: epithelial cell differentiation; cytoskeleton organization and biogenesis; negative regulation of epithelial cell proliferation

Disease: White Sponge Nevus 1

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Product Notes

The KRT4 krt4 (Catalog #AAA6484180) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The KRT4, CT (Keratin, Type II Cytoskeletal 4, Cytokeratin-4, CK-4, Keratin-4, K4, CYK4, Type-II Keratin Kb4) (MaxLight 550) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's KRT4 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:50-1:100 FLISA: 1:1,000 WB: 1:50-1:100 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the KRT4 krt4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KRT4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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