Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Zona pellucida sperm-binding protein 2 (ZP2) Standard Curve.)
Rat Zona Pellucida Sperm-Binding Protein 2 (ZP2) ELISA Kit | ZP2 elisa kit
Rat Zona Pellucida Sperm-Binding Protein 2 (ZP2) ELISA Kit
Reactivity
Rat
Synonyms
Zona Pellucida Sperm-Binding Protein 2 (ZP2); Rat Zona Pellucida Sperm-Binding Protein 2 (ZP2) ELISA Kit; ZP2; ZPA; zona pellucida glycoprotein 2; zona pellucida protein A; zona pellucida sperm-binding protein 2; ZP2 elisa kit
Reactivity
Rat
Specificity
Rat Zona pellucida sperm-binding protein 2 (ZP2) ELISA Kit has high sensitivity and excellent specificity for detection of Rat ZP2. No significant cross-reactivity or interference between Rat ZP2 and analogues was observed.
Sequence Length
666
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Zona pellucida sperm-binding protein 2 (ZP2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Zona pellucida sperm-binding protein 2 (ZP2) Standard Curve.)
Related Product Information for ZP2 elisa kit
Description: This Rat Zona pellucida sperm-binding protein 2 (ZP2) ELISA Kit employs a two-site sandwich ELISA to quantitate ZP2 in samples. An antibody specific for ZP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ZP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. Zona pellucida sperm-binding protein 2 is a structural component of the zona pellucida and functions in secondary binding and penetration of acrosome-reacted spermatozoa. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a consensus furin cleavage site, and a C-terminal transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies.
Background: The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. Zona pellucida sperm-binding protein 2 is a structural component of the zona pellucida and functions in secondary binding and penetration of acrosome-reacted spermatozoa. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a consensus furin cleavage site, and a C-terminal transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies.
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
NCBI Official Full Name
Zona pellucida sperm-binding protein 2
UniProt Protein Name
Zona pellucida sperm-binding protein 2
Protein Family
UniProt Gene Name
ZP2
UniProt Synonym Gene Names
ZPA; Zp-2
UniProt Entry Name
ZP2_RABIT
Uniprot Description
Function: The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP2 may act as a secondary sperm receptor.
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Product Notes
The Rat ZP2 zp2 (Catalog #AAA9712597) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712597 ELISA Kit recognizes Rat ZP2. It is sometimes possible for the material contained within the vial of "Zona Pellucida Sperm-Binding Protein 2 (ZP2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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