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Western Blot (WB) (Western Blot (WB) analysis of ZP2 mAb at 1:500 dilution Lane1:Hela whole cell lysate (40ug) Lane2:H9C2 whole cell lysate (40ug) Lane3:RAW264. 7 whole cell lysate (40ug) Lane4:MCF-7 whole cell lysate (40ug))

Rabbit ZP2 Polyclonal Antibody | anti-ZP2 antibody

ZP2 Polyclonal Antibody

Gene Names
ZP2; ZPA; Zp-2; OOMD6
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
ZP2; Polyclonal Antibody; ZP2 Polyclonal Antibody; Zona pellucida sperm-binding protein 2; Zona pellucida glycoprotein 2; Zp-2; Zona pellucida protein A; ZPA; anti-ZP2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
ZP2 polyclonal antibody detects endogenous levels of ZP2 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Applicable Applications for anti-ZP2 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
Synthetic peptide, corresponding to amino acids 395-440 of Human ZP2.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of ZP2 mAb at 1:500 dilution Lane1:Hela whole cell lysate (40ug) Lane2:H9C2 whole cell lysate (40ug) Lane3:RAW264. 7 whole cell lysate (40ug) Lane4:MCF-7 whole cell lysate (40ug))

Western Blot (WB) (Western Blot (WB) analysis of ZP2 mAb at 1:500 dilution Lane1:Hela whole cell lysate (40ug) Lane2:H9C2 whole cell lysate (40ug) Lane3:RAW264. 7 whole cell lysate (40ug) Lane4:MCF-7 whole cell lysate (40ug))
Related Product Information for anti-ZP2 antibody
The mammalian zona pellucida is composed of three major glycoproteins, ZP1, ZP2 and ZP3. ZP2 has been implicated as a secondary sperm receptor that binds sperm only after the induction of the sperm acrosome reaction. Both ZP2 and ZP3 are modified by the zona reaction; ZP2 undergoes a proteolytic cleavage and ZP3 loses its ability to induce the acrosome reaction and its sperm receptor activity. During the process of fertilization, the initial interaction between male and female gametes is mediated by a sperm receptor, ZP3, which resides in the extracellular glycoprotein matrix (zona pellucida) surrounding the oocyte. The sperm receptor function of the ZP3 molecule plays a key role in the first step of the fertilization process. Following sperm-oocyte binding, ZP3 triggers the sperm acrosome reaction that releases the protein machinery, enabling a spermatozoon to penetrate the zona pellucida.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
~ 82kDa
NCBI Official Synonym Full Names
zona pellucida glycoprotein 2
NCBI Official Symbol
ZP2
NCBI Official Synonym Symbols
ZPA; Zp-2; OOMD6
NCBI Protein Information
zona pellucida sperm-binding protein 2
UniProt Protein Name
Zona pellucida sperm-binding protein 2
UniProt Gene Name
ZP2
UniProt Synonym Gene Names
ZPA; Zp-2
UniProt Entry Name
ZP2_HUMAN

NCBI Description

The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed of three glycoproteins with various functions during fertilization and preimplantation development. The glycosylated mature peptide is one of the structural components of the zona pellucida and functions in secondary binding and penetration of acrosome-reacted spermatozoa. Female mice lacking this gene do not form a stable zona matrix and are sterile. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]

Uniprot Description

ZP2: The mammalian zona pellucida, which mediates species- specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP2 may act as a secondary sperm receptor. Belongs to the ZP domain family. ZPA subfamily.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 16p12

Cellular Component: endoplasmic reticulum; extracellular matrix; extracellular region; Golgi apparatus; integral to membrane; multivesicular body; plasma membrane; proteinaceous extracellular matrix; secretory granule

Molecular Function: acrosin binding; coreceptor activity

Biological Process: binding of sperm to zona pellucida; intracellular protein transport; manganese ion transport; regulation of acrosome reaction; signal transduction

Research Articles on ZP2

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Product Notes

The ZP2 zp2 (Catalog #AAA3007389) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ZP2 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ZP2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the ZP2 zp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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