Human Signaling Lymphocytic Activation Molecule ELISA Kit | SLAM/CD150 elisa kit

Human Signaling Lymphocytic Activation Molecule ELISA Kit

Cat. Num. AAA762072

• Gene Names: SLAMF1; SLAM; CD150; CDw150
• Reactivity: Human
• Synonyms: Signaling Lymphocytic Activation Molecule; Human Signaling Lymphocytic Activation Molecule ELISA Kit; IPO-3/CD150IPO-3/CDw150/signaling lymphocytic activation molecule family member 1/SLAMCD150 antigen; SLAM/CD150 elisa kit

• Reactivity: Human
• Sequence Length: 335

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for SLAM/CD150 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human SLAM/CD150 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human SLAM/CD150 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human SLAM/CD150 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human SLAM/CD150 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 984969
• NCBI GeneID: 6504
• Molecular Weight: 40,292 Da
• NCBI Official Full Name: signaling lymphocytic activation molecule
• NCBI Official Synonym Full Names: signaling lymphocytic activation molecule family member 1
• NCBI Official Symbol: SLAMF1
• NCBI Official Synonym Symbols: SLAM; CD150; CDw150
• NCBI Protein Information: signaling lymphocytic activation molecule
• UniProt Protein Name: Signaling lymphocytic activation molecule
• Protein Family: Signaling lymphocytic activation molecule
• UniProt Gene Name: SLAMF1
• UniProt Synonym Gene Names: SLAM
• UniProt Entry Name: SLAF1_HUMAN

Uniprot Description

SLAM: a type I membrane protein present on the surface of B cells, T cells and CD83+ dendritic cells. A high-affinity self-ligand considered to be important in bidirectional T <-> B-cell stimulation. SLAM-induced signal-transduction events in T lymphocytes are different from those in B cells. Two modes of SLAM signaling are likely to exist: one in which the inhibitor SH2D1A (SAP) acts as a negative regulator and another in which protein-tyrosine phosphatase 2C (SHP-2)-dependent signal transduction operates. Its cytoplasmic domain interacts with SAP through part of its SH2 domain, and with SHP-2. Three alternatively spliced isoforms have been described.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 1q23.3

Cellular Component: external side of plasma membrane; integral to membrane; phagocytic vesicle

Molecular Function: antigen binding; protein binding; transmembrane receptor activity; viral receptor activity

Biological Process: adaptive immune response; cell adhesion; entry of virus into host cell; innate immune response; lymphocyte activation; myeloid dendritic cell activation during immune response; negative regulation of interleukin-12 production; negative regulation of interleukin-6 production; negative regulation of tumor necrosis factor production; phagocytosis; positive regulation of cell proliferation; positive regulation of JNK cascade; regulation of catalytic activity; regulation of vesicle fusion; signal transduction