Rat Vasodilator Stimulated Phosphoprotein (VASP) ELISA Kit | VASP elisa kit

Rat Vasodilator Stimulated Phosphoprotein (VASP) ELISA Kit

Cat. Num. AAA2000343

• Reactivity: Rat
• Synonyms: Vasodilator Stimulated Phosphoprotein (VASP); Rat Vasodilator Stimulated Phosphoprotein (VASP) ELISA Kit; VASP elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Vasodilator Stimulated Phosphoprotein (VASP).; No significant cross-reactivity or interference between Vasodilator Stimulated Phosphoprotein (VASP) and analogues was observed.
• Sequence Length: 380

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.060ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vasodilator Stimulated Phosphoprotein (VASP) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vasodilator Stimulated Phosphoprotein (VASP) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for VASP elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vasodilator Stimulated Phosphoprotein (VASP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vasodilator Stimulated Phosphoprotein (VASP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vasodilator Stimulated Phosphoprotein (VASP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vasodilator Stimulated Phosphoprotein (VASP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for VASP elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 4507869
• NCBI GeneID: 7408
• NCBI Accession #: NP_003361.1
• NCBI GenBank Nucleotide #: NM_003370.3
• UniProt Accession #: P50552; Q6PIZ1; Q93035; B2RBT9
• Molecular Weight: 39,830 Da
• NCBI Official Full Name: vasodilator-stimulated phosphoprotein
• NCBI Official Synonym Full Names: vasodilator-stimulated phosphoprotein
• NCBI Official Symbol: VASP
• NCBI Protein Information: vasodilator-stimulated phosphoprotein
• UniProt Protein Name: Vasodilator-stimulated phosphoprotein
• Protein Family: Vasodilator-stimulated phosphoprotein
• UniProt Gene Name: VASP
• UniProt Synonym Gene Names: VASP
• UniProt Entry Name: VASP_HUMAN

NCBI Description

Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. [provided by RefSeq, Jul 2008]

Uniprot Description

VASP: vasodilator-stimulated phosphoprotein. Actin- and profilin-binding microfilament-associated protein. The phosphorylation of VASP is dynamically regulated by cellular adhesion to extracellular matrix.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: filopodium membrane; focal adhesion; tight junction; cytoplasm; plasma membrane; cytosol; actin cytoskeleton

Molecular Function: protein binding; actin binding; SH3 domain binding; profilin binding

Biological Process: axon guidance; positive regulation of actin filament polymerization; actin polymerization and/or depolymerization; neural tube closure; T cell receptor signaling pathway; protein homotetramerization