Rat Vascular Endothelial Growth Factor Receptor 3 (VEGFR3) ELISA Kit | VEGFR3 elisa kit

Rat Vascular Endothelial Growth Factor Receptor 3 (VEGFR3) ELISA Kit

Cat. Num. AAA450591

• Gene Names: Flt4; Vegfr3
• Reactivity: Rat
• Synonyms: Vascular Endothelial Growth Factor Receptor 3 (VEGFR3); Rat Vascular Endothelial Growth Factor Receptor 3 (VEGFR3) ELISA Kit; FLT41; FLT4; PCL; Fms-Related Tyrosine Kinase 4; Tyrosine-protein kinase receptor FLT4; VEGFR3 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of VEGFR3. No significant cross-reactivity or interference between VEGFR3 and analogues was observed.
• Sequence Length: 1363

• Samples: Serum, plasma, tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.064ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level VEGFR3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level VEGFR3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for VEGFR3 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of VEGFR3 in rat serum, plasma, tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to VEGFR3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to VEGFR3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain VEGFR3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of VEGFR3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 16758466
• NCBI GeneID: 114110
• NCBI Accession #: NP_446104.1
• NCBI GenBank Nucleotide #: NM_053652.1
• UniProt Accession #: Q91ZT1; O35755; Q91ZT0
• Molecular Weight: 123,178 Da
• NCBI Official Full Name: vascular endothelial growth factor receptor 3
• NCBI Official Synonym Full Names: fms-related tyrosine kinase 4
• NCBI Official Symbol: Flt4
• NCBI Official Synonym Symbols: Vegfr3
• NCBI Protein Information: vascular endothelial growth factor receptor 3
• UniProt Protein Name: Vascular endothelial growth factor receptor 3
• UniProt Gene Name: Flt4
• UniProt Synonym Gene Names: Flt-4; Vegfr3; VEGFR-3; FLT-4

NCBI Description

VEGF receptor-related, cell surface associated kinase with growth promoting ability [RGD, Feb 2006]

Uniprot Description

Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFC and VEGFD, and plays an essential role in adult lymphangiogenesis and in the development of the vascular network and the cardiovascular system during embryonic development. Promotes proliferation, survival and migration of endothelial cells, and regulates angiogenic sprouting. Signaling by activated FLT4 leads to enhanced production of VEGFC, and to a lesser degree VEGFA, thereby creating a positive feedback loop that enhances FLT4 signaling. Modulates KDR signaling by forming heterodimers. Mediates activation of the MAPK1/ERK2, MAPK3/ERK1 signaling pathway, of MAPK8 and the JUN signaling pathway, and of the AKT1 signaling pathway. Phosphorylates SHC1. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Promotes phosphorylation of MAPK8 at 'Thr-183' and 'Tyr-185', and of AKT1 at 'Ser-473' ().