Human Vascular Endothelial Growth Factor Receptor 3 (VEGFR3) RTU ELISA Kit | VEGFR3 rtu elisa kit

Human Vascular Endothelial Growth Factor Receptor 3 (VEGFR3) ELISA Kit

Cat. Num. AAA4503315

• Gene Names: FLT4; PCL; FLT-4; FLT41; LMPH1A; VEGFR3; VEGFR-3
• Reactivity: Human
• Synonyms: Vascular Endothelial Growth Factor Receptor 3 (VEGFR3); Human Vascular Endothelial Growth Factor Receptor 3 (VEGFR3) ELISA Kit; FLT41; FLT4; PCL; Fms-Related Tyrosine Kinase 4; Tyrosine-protein kinase receptor FLT4; VEGFR3 rtu elisa kit

• Reactivity: Human
• Sequence Length: 1298

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.125ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for VEGFR3 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to VEGFR3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to VEGFR3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain VEGFR3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of VEGFR3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 103472027
• NCBI GeneID: 2324
• NCBI Accession #: NP_002011.2
• NCBI GenBank Nucleotide #: NM_002020.4
• UniProt Accession #: P35916; Q16067; Q86W07; Q86W08; A8K6L4; B5A926
• Molecular Weight: 93,174 Da
• NCBI Official Full Name: vascular endothelial growth factor receptor 3 isoform 2
• NCBI Official Synonym Full Names: fms related tyrosine kinase 4
• NCBI Official Symbol: FLT4
• NCBI Official Synonym Symbols: PCL; FLT-4; FLT41; LMPH1A; VEGFR3; VEGFR-3
• NCBI Protein Information: vascular endothelial growth factor receptor 3
• UniProt Protein Name: Vascular endothelial growth factor receptor 3
• UniProt Gene Name: FLT4
• UniProt Synonym Gene Names: VEGFR3; VEGFR-3; FLT-4

NCBI Description

This gene encodes a tyrosine kinase receptor for vascular endothelial growth factors C and D. The protein is thought to be involved in lymphangiogenesis and maintenance of the lymphatic endothelium. Mutations in this gene cause hereditary lymphedema type IA. [provided by RefSeq, Jul 2008]

Uniprot Description

Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFC and VEGFD, and plays an essential role in adult lymphangiogenesis and in the development of the vascular network and the cardiovascular system during embryonic development. Promotes proliferation, survival and migration of endothelial cells, and regulates angiogenic sprouting. Signaling by activated FLT4 leads to enhanced production of VEGFC, and to a lesser degree VEGFA, thereby creating a positive feedback loop that enhances FLT4 signaling. Modulates KDR signaling by forming heterodimers. The secreted isoform 3 may function as a decoy receptor for VEGFC and/or VEGFD and play an important role as a negative regulator of VEGFC-mediated lymphangiogenesis and angiogenesis. Binding of vascular growth factors to isoform 1 or isoform 2 leads to the activation of several signaling cascades; isoform 2 seems to be less efficient in signal transduction, because it has a truncated C-terminus and therefore lacks several phosphorylation sites. Mediates activation of the MAPK1/ERK2, MAPK3/ERK1 signaling pathway, of MAPK8 and the JUN signaling pathway, and of the AKT1 signaling pathway. Phosphorylates SHC1. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Promotes phosphorylation of MAPK8 at 'Thr-183' and 'Tyr-185', and of AKT1 at 'Ser-473'.