Rat VEGFR3 ELISA Kit | FLT4 elisa kit

Rat VEGFR3 ELISA Kit

Cat. Num. AAA8244711

• Gene Names: Flt4; Vegfr3
• Reactivity: Rat
• Applications: ELISA
• Synonyms: VEGFR3; Rat VEGFR3 ELISA Kit; Vascular endothelial growth factor receptor 3; VEGFR-3; Fms-like tyrosine kinase 4; FLT-4; Tyrosine-protein kinase receptor FLT4; FLT4 elisa kit

• Reactivity: Rat
• Specificity: The Rat VEGFR3 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Rat VEGFR3 proteins within the range of 156 pg/ml - 10000 pg/ml.
• Sequence Length: 1363

• Applicable Applications for FLT4 elisa kit: Sandwich ELISA (EIA)
• Samples: Cell Culture Supernatants, Serum.
• Sensitivity: 10 pg/ml.
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve

Related Product Information for FLT4 elisa kit

Principle of the Assay||The Rat VEGFR3 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat VEGFR3 in Cell Culture Supernatants, Serum. This assay employs an antibody specific for Rat VEGFR3 coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGFR3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat VEGFR3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGFR3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background/Introduction: Fms-related tyrosine kinase 4, also known as FLT4 or VEGFR3, is a protein which in humans is encoded by the FLT4 gene. It is mapped to 5q35.3. This gene encodes a tyrosine kinase receptor for vascular endothelial growth factors C and D. The protein is thought to be involved in lymphangiogenesis and maintenance of the lymphatic endothelium. FLT4 has an essential role in the development of the embryonic cardiovascular system before the emergence of the lymphatic vessels. It has been found that FLT4, which provides proangiogenic signaling when expressed on endothelium, may also have antiangiogenic properties when expressed at an avascular site by nonendothelial cells. FLT4 is also regarded as a regulator of vascular network formation.

NCBI and Uniprot Product Information

• NCBI GI #: 81867516
• NCBI GeneID: 114110
• UniProt Accession #: Q91ZT1; O35755; Q91ZT0
• Molecular Weight: 123,178 Da
• NCBI Official Full Name: Vascular endothelial growth factor receptor 3
• NCBI Official Synonym Full Names: fms-related tyrosine kinase 4
• NCBI Official Symbol: Flt4
• NCBI Official Synonym Symbols: Vegfr3
• NCBI Protein Information: vascular endothelial growth factor receptor 3
• UniProt Protein Name: Vascular endothelial growth factor receptor 3
• Protein Family: Vascular endothelial growth factor receptor
• UniProt Gene Name: Flt4
• UniProt Synonym Gene Names: Flt-4; Vegfr3; VEGFR-3; FLT-4
• UniProt Entry Name: VGFR3_RAT

NCBI Description

VEGF receptor-related, cell surface associated kinase with growth promoting ability [RGD, Feb 2006]

Uniprot Description

VEGFR3: Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFC and VEGFD, and plays an essential role in adult lymphangiogenesis and in the development of the vascular network and the cardiovascular system during embryonic development. Promotes proliferation, survival and migration of endothelial cells, and regulates angiogenic sprouting. Signaling by activated FLT4 leads to enhanced production of VEGFC, and to a lesser degree VEGFA, thereby creating a positive feedback loop that enhances FLT4 signaling. Modulates KDR signaling by forming heterodimers. The secreted isoform 3 may function as a decoy receptor for VEGFC and/or VEGFD and play an important role as a negative regulator of VEGFC-mediated lymphangiogenesis and angiogenesis. Binding of vascular growth factors to isoform 1 or isoform 2 leads to the activation of several signaling cascades; isoform 2 seems to be less efficient in signal transduction, because it has a truncated C-terminus and therefore lacks several phosphorylation sites. Mediates activation of the MAPK1/ERK2, MAPK3/ERK1 signaling pathway, of MAPK8 and the JUN signaling pathway, and of the AKT1 signaling pathway. Phosphorylates SHC1. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3- kinase. Promotes phosphorylation of MAPK8 at 'Thr-183' and 'Tyr- 185', and of AKT1 at 'Ser-473'. Interacts with VEGFC and VEGFD. Monomer in the absence of bound VEGFC or VEGFD. Homodimer in the presence of bound VEGFC or VEGFD. Can also form a heterodimer with KDR. Interacts with PTPN14; the interaction is enhanced by stimulation with VEGFC. Interacts with CRK, GRB2, PTK2/FAK1, SHC1, PIK3R1 and PTPN11/SHP- 2. Identified in a complex with SRC and ITGB1. Detected in endothelial cells. Widely expressed. Detected in fetal spleen, lung and brain. Detected in adult liver, muscle, thymus, placenta, lung, testis, ovary, prostate, heart, and kidney. Present in an inactive conformation in the absence of bound ligand. Binding of VEGFC or VEGFD leads to dimerization and activation by autophosphorylation on tyrosine residues. Inhibited by MAZ51. Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.10.1; Protein kinase, tyrosine (receptor); Protein kinase, TK; Membrane protein, integral; Kinase, protein; TK group; VEGFR family

Cellular Component: cytoplasm; integral to plasma membrane; nucleus; plasma membrane; receptor complex

Molecular Function: ATP binding; growth factor binding; protein phosphatase binding; transmembrane receptor protein tyrosine kinase activity; vascular endothelial growth factor receptor activity

Biological Process: alveolus development; blood vessel morphogenesis; lymph vessel development; lymphangiogenesis; negative regulation of apoptosis; peptidyl-tyrosine phosphorylation; positive regulation of cell growth; positive regulation of cell proliferation; positive regulation of endothelial cell proliferation; positive regulation of JNK cascade; positive regulation of MAPKKK cascade; positive regulation of protein amino acid phosphorylation; protein amino acid autophosphorylation; respiratory gaseous exchange; respiratory system process; sprouting angiogenesis; vascular endothelial growth factor receptor signaling pathway; vasculature development