V-Jun Sarcoma Virus 17 Oncogene Homolog Recombinant Protein | JUN recombinant protein

Recombinant V-Jun Sarcoma Virus 17 Oncogene Homolog (JUN)

Cat. Num. AAA2031255

• Gene Names: JUN; AP1; AP-1; c-Jun
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: V-Jun Sarcoma Virus 17 Oncogene Homolog; Recombinant V-Jun Sarcoma Virus 17 Oncogene Homolog (JUN); JUN recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Concentration: 6.4 (varies by lot)
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- DDALNASFLP SESGPYGYSN PKILKQSMTL NLADPVGSLK PHLRAKNSDL LTSPDVGLLK LASPELERLI IQSSNGHITT TPTPTQFLCP KNVTDEQEGF AEGFVRALAE LHSQNTLPSV TSAAQPVNGA GMVAPAVASV AGGSGSGGFS ASLHSEPPVY ANLSNFNPGA LSSGGGAPSY GAAGLAFPAQ PQQQQQPPHH LPQQMPVQHP RLQALKEEPQ TVPEMPGETP PLSPIDMESQERIKAERKRM RN
• Sequence Length: 331

• Applicable Applications for JUN recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 30.4kDa
• Accurate Molecular Mass (KD): 34kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and T7-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Asp11~Asn262
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 119627033
• NCBI GeneID: 3725
• Molecular Weight: 35,676 Da
• NCBI Official Full Name: v-jun sarcoma virus 17 oncogene homolog (avian)
• NCBI Official Synonym Full Names: jun proto-oncogene
• NCBI Official Symbol: JUN
• NCBI Official Synonym Symbols: AP1; AP-1; c-Jun
• NCBI Protein Information: transcription factor AP-1
• UniProt Protein Name: Transcription factor AP-1
• Protein Family: Viral jun-transforming protein
• UniProt Gene Name: JUN
• UniProt Synonym Gene Names: AP1
• UniProt Entry Name: JUN_HUMAN

NCBI Description

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq, Jul 2008]

Uniprot Description

Jun: the protooncogene c-Jun is a component of the transcription factor AP-1, which binds and activates transcription at TRE/AP-1 elements. The Jun Kinases (JNKs) binds to the N-terminal region of c-Jun and phosphorylate it in response to extracellular signals including growth factors, cytokines and stress, regulating its transcriptional activity.

Protein type: Transcription factor; Oncoprotein

Chromosomal Location of Human Ortholog: 1p32-p31

Cellular Component: nucleoplasm; nuclear chromosome; transcription factor complex; transcriptional repressor complex; cytosol; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; enzyme binding; DNA binding; double-stranded DNA binding; transcription coactivator activity; transcription factor activity; transcription factor binding

Biological Process: positive regulation of monocyte differentiation; transcription from RNA polymerase II promoter; circadian rhythm; response to cAMP; regulation of cell cycle; positive regulation of smooth muscle cell proliferation; positive regulation of transcription, DNA-dependent; stress-activated MAPK cascade; microglial cell activation; response to lipopolysaccharide; SMAD protein nuclear translocation; toll-like receptor 3 signaling pathway; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; negative regulation of cell proliferation; positive regulation of fibroblast proliferation; response to radiation; regulation of transcription factor activity; transforming growth factor beta receptor signaling pathway; positive regulation of neuron apoptosis; negative regulation of protein amino acid autophosphorylation; negative regulation of neuron apoptosis; angiogenesis; toll-like receptor 4 signaling pathway; aging; response to drug; release of cytochrome c from mitochondria; monocyte differentiation; leading edge cell differentiation; axon regeneration; MyD88-independent toll-like receptor signaling pathway; learning; liver development; negative regulation of DNA binding; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; membrane depolarization; cellular response to potassium ion starvation; response to hydrogen peroxide; response to mechanical stimulus; response to cytokine stimulus; toll-like receptor signaling pathway; innate immune response; positive regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; negative regulation of transcription, DNA-dependent; positive regulation of DNA replication