PSMA6 recombinant protein

PSMA6, 1-246aa, Human, His tag, E Coli

Cat. Num. AAA204346

• Gene Names: PSMA6; IOTA; p27K; PROS27
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: PSMA6; 1-246aa; Human; His tag; E Coli; Proteasome subunit alpha type 6; IOTA; p27K; PROS27; PSMA6 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 40% glycerol, 0.1M NaCl
• Concentration: 0.25 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMSRGSS AGFDRHITIF SPEGRLYQVE YAFKAINQGG LTSVAVRGKD CAVIVTQKKV PDKLLDSSTV THLFKITENI GCVMTGMTAD SRSQVQRARY EAANWKYKYG YEIPVDMLCK RIADISQVYT QNAEMRPLGC CMILIGIDEE QGPQVYKCDP AGYYCGFKAT AAGVKQTEST SFLEKKVKKK FDWTFEQTVE TAITCLSTVL SIDFKPSEIE VGVVTVENPK FRILTEAEID AHLVALAERD
• Sequence Length: 246

• Applicable Applications for PSMA6 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for PSMA6 recombinant protein

Proteasome subunit alpha type 6, also known as PSMA6, is a member of the peptidase T1A family, which is a 20S core alpha subunit. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. PSMA6 is distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. Recombinant human PSMA6 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for PSMA6 recombinant protein

Ubiquitination

References

Nandi D., et al. (2006) J Biosci. 31:137-155.; Kristensen P., et al. (1995) Biochem Biophys Res Commun. 205:1785-1789.;

NCBI and Uniprot Product Information

• NCBI GI #: 23110944
• NCBI GeneID: 5687
• NCBI Accession #: NP_002782
• NCBI GenBank Nucleotide #: NM_002791.2
• UniProt Accession #: P60900; P34062; Q6IB60; B2R7J9; B4DQR4; B4DXJ9
• Molecular Weight: 29.9 kDa (270aa), confirmed by MALDI-TOF
• NCBI Official Full Name: proteasome subunit alpha type-6 isoform a
• NCBI Official Synonym Full Names: proteasome subunit alpha 6
• NCBI Official Symbol: PSMA6
• NCBI Official Synonym Symbols: IOTA; p27K; PROS27
• NCBI Protein Information: proteasome subunit alpha type-6
• UniProt Protein Name: Proteasome subunit alpha type-6
• Protein Family: Proteasome
• UniProt Gene Name: PSMA6
• UniProt Synonym Gene Names: PROS27; PROS-27; p27K

NCBI Description

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Multiple transcript variants encoding several different isoforms have been found for this gene. A pseudogene has been identified on the Y chromosome. [provided by RefSeq, Aug 2013]

Uniprot Description

Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).