Typical Testing Data/Standard Curve (for reference only)
()
Rat Ubiquitin (Ub) ELISA Kit | Ub elisa kit
Rat Ubiquitin (Ub) ELISA Kit
Reactivity
Rat
Synonyms
Ubiquitin (Ub); Rat Ubiquitin (Ub) ELISA Kit; Ub elisa kit
Reactivity
Rat
Sequence Length
128
Assay Type
Sandwich
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
()
Typical Testing Data/Standard Curve (for reference only)
()
Related Product Information for Ub elisa kit
Principle of the Assay: Rat Ubiquitin (Ub) ELISA Kit employs a two-site sandwich ELISA to quantitate Ub in samples. An antibody specific for Ub has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Ub present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Rat Ub detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of Ub bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background/Introduction: Ubiquitin is a small (8.6 kDa) regulatory protein found in most tissues of eukaryotic organisms. The addition of ubiquitin to a substrate protein is called ubiquitylation (or, alternatively, ubiquitination or ubiquitinylation). Ubiquitylation affects proteins in many ways: it can mark them for degradation via the proteasome, alter their cellular location, affect their activity, and promote or prevent protein interactions. Ubiquitin had been isolated from thymus prior to its identification as APF-1.10 The evidence available at the time indicated that it was a universal constituent of living cells.
Background/Introduction: Ubiquitin is a small (8.6 kDa) regulatory protein found in most tissues of eukaryotic organisms. The addition of ubiquitin to a substrate protein is called ubiquitylation (or, alternatively, ubiquitination or ubiquitinylation). Ubiquitylation affects proteins in many ways: it can mark them for degradation via the proteasome, alter their cellular location, affect their activity, and promote or prevent protein interactions. Ubiquitin had been isolated from thymus prior to its identification as APF-1.10 The evidence available at the time indicated that it was a universal constituent of living cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
ubiquitin
Similar Products
Product Notes
The Rat Ub (Catalog #AAA9711627) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711627 ELISA Kit recognizes Rat Ub. It is sometimes possible for the material contained within the vial of "Ubiquitin (Ub), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
