Western Blot (WB)
(RPA4 polyclonal antibody. Western Blot analysis of RPA4 expression in human spleen.)
Mouse anti-Human RPA4 Polyclonal Antibody | anti-RPA4 antibody
RPA4 (Replication Factor A Protein 4, RF-A Protein 4, Replication Protein A 30 kDa Subunit, RP-A p30, HSU24186)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: Immunofluorescence: 10ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml
Western Blot (WB)
(RPA4 polyclonal antibody. Western Blot analysis of RPA4 expression in human spleen.)
Western Blot (WB)
(RPA4 polyclonal antibody. Western Blot analysis of RPA4 expression in human spleen.)
Western Blot (WB)
(Western Blot analysis of RPA4 expression in transfected 293T cell line by RPA4 polyclonal antibody. Lane 1: RPA4 transfected lysate (28.71kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of RPA4 expression in transfected 293T cell line by RPA4 polyclonal antibody. Lane 1: RPA4 transfected lysate (28.71kD). Lane 2: Non-transfected lysate.)
Immunohistochemistry (IHC)
(Immunoperoxidase of purified antibody to RPA4 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml].)
Immunohistochemistry (IHC)
(Immunoperoxidase of purified antibody to RPA4 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml].)
Immunofluorescence (IF)
(Immunofluorescence of purified antibody to RPA4 on HeLa cell. [antibody concentration 10ug/ml].)
Immunofluorescence (IF)
(Immunofluorescence of purified antibody to RPA4 on HeLa cell. [antibody concentration 10ug/ml].)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a single-stranded DNA-binding protein that is the 30-kDa subunit of the replication protein A complex. Replication protein A is an essential factor for DNA double-strand break repair and cell cycle checkpoint activation. The encoded protein localizes to DNA repair foci and may be involved in the cellular DNA damage response. This protein may also play a role in inhibiting viral replication.[provided by RefSeq, Apr 2010]
Uniprot Description
RPA4: functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange. Component of the alternative replication protein A complex (aRPA) composed of RPA1, RPA3 and RPA4. Interacts with RPA1 and RPA3. Localizes to DNA repair foci after DNA damage.
Chromosomal Location of Human Ortholog: Xq21.33
Cellular Component: nucleoplasm; DNA replication factor A complex; nucleus
Molecular Function: single-stranded DNA binding
Biological Process: DNA replication initiation; nucleotide-excision repair; DNA damage checkpoint; DNA replication during S phase; mitotic cell cycle; G1/S transition of mitotic cell cycle; DNA recombination
Research Articles on RPA4
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Product Notes
The RPA4 rpa4 (Catalog #AAA644897) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The RPA4 (Replication Factor A Protein 4, RF-A Protein 4, Replication Protein A 30 kDa Subunit, RP-A p30, HSU24186) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RPA4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence. Dilution: Immunofluorescence: 10ug/ml Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml. Researchers should empirically determine the suitability of the RPA4 rpa4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MSKSGFGSYG SISAADGASG GSDQLCERDA TPAIKTQRPK VRIQDVVPCN VNQLLSSTVF DPVFKVRGII VSQVSIVGVI RGAEKASNHI CYKIDDMTAK PIEARQWFGR EKVKQVTPLS VGVYVKVFGI LKCPTGTKSL EVLKIHVLED MNEFTVHILE TVNAHMMLDK ARRDTTVESV PVSPSEVNDA GDNDESHRNF IQDEVLRLIH ECPHQEGKSI HELRAQLCDL SVKAIKEAID YLTVEGHIYP TVDREHFKSA D. It is sometimes possible for the material contained within the vial of "RPA4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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