Rat REST (RE1-Silencing Transcription Factor) ELISA Kit | REST elisa kit

Rat REST (RE1-Silencing Transcription Factor)

Cat. Num. AAA766205

• Gene Names: REST; WT6; XBR; NRSF
• Reactivity: Rat
• Synonyms: REST (RE1-Silencing Transcription Factor); Rat REST (RE1-Silencing Transcription Factor); REST elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of REST . No significant cross-reactivity or interference between REST and analogues was observed.
• Sequence Length: 1097

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 31.2-2000pg/ml
• Sensitivity: 10pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level REST were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level REST were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: 4 degree C for 6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for REST elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- REST antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-REST antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the REST amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of REST can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 301897977
• NCBI GeneID: 5978
• NCBI Accession #: NP_001180437.1
• NCBI GenBank Nucleotide #: NM_001193508.1
• Molecular Weight: 119,083 Da
• NCBI Official Full Name: RE1-silencing transcription factor
• NCBI Official Synonym Full Names: RE1 silencing transcription factor
• NCBI Official Symbol: REST
• NCBI Official Synonym Symbols: WT6; XBR; NRSF
• NCBI Protein Information: RE1-silencing transcription factor
• UniProt Protein Name: RE1-silencing transcription factor
• Protein Family: RE1-silencing transcription factor
• UniProt Gene Name: REST
• UniProt Synonym Gene Names: NRSF; XBR
• UniProt Entry Name: REST_HUMAN

NCBI Description

This gene encodes a transcriptional repressor that represses neuronal genes in non-neuronal tissues. It is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells and it is thought that this repressor may act as a master negative regular of neurogenesis. Alternatively spliced transcript variants have been described [provided by RefSeq, Jul 2010]

Uniprot Description

REST: a transcriptional repressor that binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells during embryonic development. Induced during ageing in human cortical and hippocampal neurons, where it represses genes that promote cell death and induces the expression of stress response genes. Protects neurons from amyloid beta- and oxidative stress-induced toxicity. The deletion of REST in the mouse brain induces age-related neurodegeneration. Restricts the expression of neuronal genes by associating with two distinct corepressors, SIN3A and RCOR1, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. REST activates DYRK1A transcription via a neuron-restricted silencer element in the DYRK1A promoter. Four isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation; C2H2-type zinc finger protein; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 4q12

Cellular Component: chromatin; cytosol; nucleoplasm; nucleus; transcriptional repressor complex

Molecular Function: chromatin binding; histone deacetylase activity; metal ion binding; outward rectifier potassium channel activity; protein binding; protein complex binding; transcription factor activity; transcription factor binding

Biological Process: hemopoietic progenitor cell differentiation; negative regulation of aldosterone biosynthetic process; negative regulation of calcium ion-dependent exocytosis; negative regulation of cell proliferation; negative regulation of insulin secretion; negative regulation of neurogenesis; negative regulation of neuron differentiation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of apoptosis; positive regulation of caspase activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of transcription, DNA-dependent; transcription from RNA polymerase II promoter