Rat Tryptophan Hydroxylase 2 (TPH2) ELISA Kit | TPH2 elisa kit

Rat Tryptophan Hydroxylase 2 (TPH2) ELISA Kit

Cat. Num. AAA2706084

• Gene Names: TPH2; NTPH; ADHD7
• Reactivity: Rat
• Synonyms: Tryptophan Hydroxylase 2 (TPH2); Rat Tryptophan Hydroxylase 2 (TPH2) ELISA Kit; NTPH; Neuronal tryptophan hydroxylase; Tryptophan 5-monooxygenase 2; TPH2 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Tryptophan Hydroxylase 2 (TPH2).; No significant cross-reactivity or interference between Tryptophan Hydroxylase 2 (TPH2) and analogues was observed.
• Sequence Length: 492

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 1.56-100ng/mL
• Sensitivity: < 0.56ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tryptophan Hydroxylase 2 (TPH2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tryptophan Hydroxylase 2 (TPH2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TPH2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tryptophan Hydroxylase 2 (TPH2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tryptophan Hydroxylase 2 (TPH2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tryptophan Hydroxylase 2 (TPH2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tryptophan Hydroxylase 2 (TPH2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 109731493
• NCBI GeneID: 121278
• UniProt Accession #: Q8IWU9; Q14CB0; A6NGA4
• Molecular Weight: 56,242 Da
• NCBI Official Full Name: Tryptophan hydroxylase 2
• NCBI Official Synonym Full Names: tryptophan hydroxylase 2
• NCBI Official Symbol: TPH2
• NCBI Official Synonym Symbols: NTPH; ADHD7
• NCBI Protein Information: tryptophan 5-hydroxylase 2
• UniProt Protein Name: Tryptophan 5-hydroxylase 2
• Protein Family: Tryptophan 5-hydroxylase
• UniProt Gene Name: TPH2
• UniProt Synonym Gene Names: NTPH

NCBI Description

This gene encodes a member of the pterin-dependent aromatic acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. Mutations in this gene may be associated with psychiatric diseases such as bipolar affective disorder and major depression. [provided by RefSeq, Feb 2016]