Human Tryptophan 5-hydroxylase 2 ELISA Kit | TPH2 elisa kit

Human Tryptophan 5-hydroxylase 2 ELISA Kit

Cat. Num. AAA765795

• Gene Names: TPH2; NTPH; ADHD7
• Reactivity: Human
• Synonyms: Tryptophan 5-hydroxylase 2; Human Tryptophan 5-hydroxylase 2 ELISA Kit; TPH2/Neuronal tryptophan hydroxylase/Tryptophan 5-monooxygenase 2/; TPH2 elisa kit

• Reactivity: Human
• Sequence Length: 490

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.312-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for TPH2 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human TPH2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human TPH2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human TPH2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human TPH2 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 31795563
• NCBI GeneID: 121278
• NCBI Accession #: NP_775489.2
• NCBI GenBank Nucleotide #: NM_173353.3
• Molecular Weight: 56,242 Da
• NCBI Official Full Name: tryptophan 5-hydroxylase 2
• NCBI Official Synonym Full Names: tryptophan hydroxylase 2
• NCBI Official Symbol: TPH2
• NCBI Official Synonym Symbols: NTPH; ADHD7
• NCBI Protein Information: tryptophan 5-hydroxylase 2
• UniProt Protein Name: Tryptophan 5-hydroxylase 2
• Protein Family: Tryptophan 5-hydroxylase
• UniProt Gene Name: TPH2
• UniProt Synonym Gene Names: NTPH
• UniProt Entry Name: TPH2_HUMAN

NCBI Description

This gene encodes a member of the pterin-dependent aromatic acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. Mutations in this gene may be associated with psychiatric diseases such as bipolar affective disorder and major depression. [provided by RefSeq, Feb 2016]

Uniprot Description

TPH2: an enzyme that is rate-limiting in the biosynthesis of serotonin. It catalyzes the biopterin dependent monooxygenation of tryptophan to 5-hydroxytryptophan (5HT), which is subsequently decarboxylated to form the neurotransmitter serotonin. Its expression is limited to a few specialized tissues: raphe neurons, pinealocytes, mast cells, mononuclear leukocytes, beta-cells of the islets of Langerhans, and intestinal and pancreatic enterochromaffin cells. Two alternatively spliced isoforms have been described. Isoform 2 appears to be expressed exclusively in the brain, and is expressed at significantly higher levels in the raphe nuclei than isoform 1.

Protein type: Oxidoreductase; Amino Acid Metabolism - tryptophan; EC 1.14.16.4

Chromosomal Location of Human Ortholog: 12q21.1

Cellular Component: cytosol; neuron projection

Molecular Function: amino acid binding; iron ion binding; tryptophan 5-monooxygenase activity

Biological Process: aromatic amino acid family metabolic process; circadian rhythm; indolalkylamine biosynthetic process; response to activity; response to calcium ion; response to estrogen stimulus; response to glucocorticoid stimulus; response to nutrient levels; serotonin biosynthetic process

Disease: Attention Deficit-hyperactivity Disorder, Susceptibility To, 7; Major Depressive Disorder