Rat Troponin C, skeletal muscle (TNNC2) ELISA Kit | TNNC2 elisa kit
Rat Troponin C, skeletal muscle (TNNC2) ELISA Kit
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat TNNC2 antibody. TNNC2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat TNNC2 Antibody is added and binds to TNNC2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated TNNC2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat TNNC2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI Description
Troponin (Tn), a key protein complex in the regulation of striated muscle contraction, is composed of 3 subunits. The Tn-I subunit inhibits actomyosin ATPase, the Tn-T subunit binds tropomyosin and Tn-C, while the Tn-C subunit binds calcium and overcomes the inhibitory action of the troponin complex on actin filaments. The protein encoded by this gene is the Tn-C subunit. [provided by RefSeq, Jul 2008]
Uniprot Description
Troponin is the central regulatory protein of striated muscle contraction. Tn consists of three components: Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments.
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Product Notes
The Rat TNNC2 tnnc2 (Catalog #AAA1600539) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1600539 ELISA Kit recognizes Rat TNNC2. It is sometimes possible for the material contained within the vial of "Troponin C, skeletal muscle (TNNC2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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