Rabbit BCL2L2 Polyclonal Antibody | anti-BCL2L2 antibody

Anti-BCL2L2 antibody

Cat. Num. AAA176073

• Gene Names: BCL2L2; BCLW; BCL-W; PPP1R51; BCL2-L-2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen affinity purified.
• Synonyms: BCL2L2; Polyclonal Antibody; Anti-BCL2L2 antibody; Bcl-2-like protein 2(Bcl2-L-2); BCL2-like 2; Apoptosis regulator Bcl-W; anti-BCL2L2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 193

• Applicable Applications for anti-BCL2L2 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human BCL2L2 (74-88aa SAQQRFTQVSDELFQ), identical to the related rat and mouse sequences.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- BCL2L2 antibody, MBS176073, Western blottingAll lanes: Anti BCL2L2 (MBS176073) at 0.5ug/mlWB: HELAWhole Cell Lysate at 40ugPredicted bind size: 21KDObserved bind size: 21KD )

Immunohistochemistry (IHC)

(Anti- BCL2L2 antibody, MBS176073,IHC(P)IHC(P): Human Rectal Cancer Tissue )

Related Product Information for anti-BCL2L2 antibody

Description: Rabbit IgG polyclonal antibody for Bcl-2-like protein 2(BCL2L2) detection. Tested with WB, IHC-P in Human, Mouse, Rat.
Background: Bcl-2-like protein 2 is a protein that in humans is encoded by the BCL2L2 gene. This gene encodes a pro-survival(anti-apoptotic) member of the bcl-2 protein family. Its gene is mapped to human chromosome 14q11.2-q12. In all, it has got 193 amino acids. Generally speaking, the BCL2 can be found in brain, colon and salivary gland. Acting as the anti- and pro-apoptotic regulators, it has played a vital role in reducing the cell apoptosis under cytotoxic conditions.

NCBI and Uniprot Product Information

• NCBI GI #: 296434404
• NCBI GeneID: 599
• UniProt Accession #: Q92843; Q2M3U0; Q5U0H4; A8K0F4
• Molecular Weight: 37,171 Da
• NCBI Official Full Name: Bcl-2-like protein 2
• NCBI Official Synonym Full Names: BCL2-like 2
• NCBI Official Symbol: BCL2L2
• NCBI Official Synonym Symbols: BCLW; BCL-W; PPP1R51; BCL2-L-2
• NCBI Protein Information: bcl-2-like protein 2; apoptosis regulator BCL-W; protein phosphatase 1, regulatory subunit 51
• UniProt Protein Name: Bcl-2-like protein 2
• Protein Family: Bcl-2-like protein
• UniProt Gene Name: BCL2L2
• UniProt Synonym Gene Names: BCLW; KIAA0271; Bcl2-L-2
• UniProt Entry Name: B2CL2_HUMAN

NCBI Description

This gene encodes a member of the BCL-2 protein family. The proteins of this family form hetero- or homodimers and act as anti- and pro-apoptotic regulators. Expression of this gene in cells has been shown to contribute to reduced cell apoptosis under cytotoxic conditions. Studies of the related gene in mice indicated a role in the survival of NGF- and BDNF-dependent neurons. Mutation and knockout studies of the mouse gene demonstrated an essential role in adult spermatogenesis. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream PABPN1 (poly(A) binding protein, nuclear 1) gene. [provided by RefSeq, Dec 2010]

Uniprot Description

BCL2L2: Promotes cell survival. Blocks dexamethasone-induced apoptosis. Mediates survival of postmitotic Sertoli cells by suppressing death-promoting activity of BAX. Expressed in a wide range of tissues with highest levels in brain, spinal cord, testis, pancreas, heart, spleen and mammary glands. Moderate levels found in thymus, ovary and small intestine. Not detected in salivary gland, muscle or liver. Also expressed in cell lines of myeloid, fibroblast and epithelial origin. Not detected in most lymphoid cell lines. Belongs to the Bcl-2 family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 14q11.2-q12

Cellular Component: mitochondrial outer membrane; cytosol

Molecular Function: BH domain binding; protein binding; protein homodimerization activity; protein heterodimerization activity

Biological Process: DNA damage response, signal transduction resulting in induction of apoptosis; Sertoli cell proliferation; spermatogenesis; negative regulation of apoptosis