Human Troponin C Type 2 ELISA Kit | TNNC2 elisa kit

Human Troponin C Type 2 ELISA Kit

Cat. Num. AAA762867

• Reactivity: Human
• Synonyms: Troponin C Type 2; Human Troponin C Type 2 ELISA Kit; TNNC2; TNNC2 elisa kit

• Reactivity: Human
• Sequence Length: 160

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 31.25-2000pg/ml
• Sensitivity: 18.75pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for TNNC2 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human TNNC2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human TNNC2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human TNNC2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human TNNC2 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 13529089
• NCBI GeneID: 7125
• Molecular Weight: 18,122 Da
• NCBI Official Full Name: Troponin C type 2 (fast)
• NCBI Official Synonym Full Names: troponin C2, fast skeletal type
• NCBI Official Symbol: TNNC2
• NCBI Protein Information: troponin C, skeletal muscle
• UniProt Protein Name: Troponin C, skeletal muscle
• Protein Family: Troponin
• UniProt Gene Name: TNNC2
• UniProt Entry Name: TNNC2_HUMAN

NCBI Description

Troponin (Tn), a key protein complex in the regulation of striated muscle contraction, is composed of 3 subunits. The Tn-I subunit inhibits actomyosin ATPase, the Tn-T subunit binds tropomyosin and Tn-C, while the Tn-C subunit binds calcium and overcomes the inhibitory action of the troponin complex on actin filaments. The protein encoded by this gene is the Tn-C subunit. [provided by RefSeq, Jul 2008]

Uniprot Description

TNNC2: Troponin is the central regulatory protein of striated muscle contraction. Tn consists of three components: Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments. Belongs to the troponin C family.

Protein type: Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 20q12-q13.11

Cellular Component: cytosol; troponin complex

Molecular Function: actin binding; calcium ion binding; protein binding

Biological Process: muscle filament sliding; regulation of muscle contraction; skeletal muscle contraction