Rat Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) ELISA Kit | TIMP2 elisa kit

Rat Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) Wide-range ELISA Kit

Cat. Num. AAA2709259

• Reactivity: Rat
• Applications: ELISA
• Synonyms: Tissue Inhibitors Of Metalloproteinase 2 (TIMP2); Rat Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) Wide-range ELISA Kit; Tissue Inhibitors Of Metalloproteinase 2; CSC-21K; TIMP Metallopeptidase Inhibitor 2; TIMP2 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Wide-range Tissue Inhibitors Of Metalloproteinase 2 (TIMP2).; No significant cross-reactivity or interference between Wide-range Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) and analogues was observed.
• Sequence Length: 220

• Applicable Applications for TIMP2 elisa kit: ELISA (EIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 1.56-100ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.59ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wide-range Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wide-range Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wide-range Tissue Inhibitors Of Metalloproteinase 2 (TIMP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Wide-range Tissue Inhibitors Of Metalloproteinase 2 (TIMP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Wide-range Tissue Inhibitors Of Metalloproteinase 2 (TIMP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Wide-range Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 619233
• NCBI GeneID: 29543
• UniProt Accession #: P30121
• NCBI Official Full Name: tissue inhibitor of metalloproteinase type 2
• NCBI Official Synonym Full Names: TIMP metallopeptidase inhibitor 2
• NCBI Official Symbol: Timp2
• NCBI Protein Information: metalloproteinase inhibitor 2
• UniProt Protein Name: Metalloproteinase inhibitor 2
• Protein Family: Metalloproteinase inhibitor
• UniProt Gene Name: Timp2
• UniProt Synonym Gene Names: Timp-2; TIMP-2

NCBI Description

acts as an inhibitor of metallopeptidase activity; may play a role in tissue remodeling during spermatogenesis [RGD, Feb 2006]

Uniprot Description

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor.