Typical Testing Data/Standard Curve (for reference only)
Rat TIMP-1 ELISA Kit | TIMP-1 elisa kit
TIMP-1 Rat ELISA Kit
Reactivity
Rat
Applications
ELISA
Synonyms
TIMP-1; TIMP-1 Rat ELISA Kit; Rat TIMP-1; TIMP-1 elisa kit
Reactivity
Rat
Applicable Applications for TIMP-1 elisa kit
Quantitative sELISA (EIA)
Application Notes
For quantitative detection of TIMP-1 in Rat serum, plasma, body fluids, tissue lysates or cell culture supernatants.
Assay Type
Sandwich
Detection Range
31.2 pg/ml-2000 pg/ml
Sensitivity
< 3pg/ml
Preparation and Storage
Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for TIMP-1 elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-TIMP-1 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-TIMP-1 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TIMP-1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of TIMP-1 can be calculated.
Background: Tissue inhibitor of metalloproteinase 1 (TIMP1), also known as Erythroid Potentiating Activity (EPA), is a 28,000 MW glycoprotein that is expressed from the several tissues of organisms. This protein is a member of the TIMP family. The glycoprotein is a natural inhibitor of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. It plays a major role in modulating the activity of interstitial collagenase as well as a number of connective tissue metalloendoproteases. Decreased TIMP1 and increased cathepsin V/L2 levels might play a role in the matrix degradation that is a hallmark of keratoconus corneas. It is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function.
Background: Tissue inhibitor of metalloproteinase 1 (TIMP1), also known as Erythroid Potentiating Activity (EPA), is a 28,000 MW glycoprotein that is expressed from the several tissues of organisms. This protein is a member of the TIMP family. The glycoprotein is a natural inhibitor of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. It plays a major role in modulating the activity of interstitial collagenase as well as a number of connective tissue metalloendoproteases. Decreased TIMP1 and increased cathepsin V/L2 levels might play a role in the matrix degradation that is a hallmark of keratoconus corneas. It is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
23,032 Da
NCBI Official Full Name
TIMP-1
NCBI Official Synonym Full Names
TIMP metallopeptidase inhibitor 1<
NCBI Official Symbol
TIMP1
NCBI Protein Information
metalloproteinase inhibitor 1; EG-1; TIMP-1; embryogenin-1; tissue inhibitor of metalloproteinases 1; tissue inhibitor of metalloproteinase 1 (erythroid potentiating activity, collagenase inhibitor)
UniProt Protein Name
Metalloproteinase inhibitor 1
UniProt Gene Name
TIMP1
UniProt Synonym Gene Names
EG-1; TIMP-1
UniProt Entry Name
TIMP1_BOVIN
Uniprot Description
Function: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor.
Subcellular location: Secreted.
Post-translational modification: The activity of TIMP1 is dependent on the presence of disulfide bonds.
Sequence similarities: Belongs to the protease inhibitor I35 (TIMP) family. [View classification]Contains 1 NTR domain.
Research Articles on TIMP-1
Similar Products
Product Notes
The Rat TIMP-1 timp1 (Catalog #AAA355282) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA355282 ELISA Kit recognizes Rat TIMP-1. AAA Biotech's TIMP-1 can be used in a range of immunoassay formats including, but not limited to, Quantitative sELISA (EIA). For quantitative detection of TIMP-1 in Rat serum, plasma, body fluids, tissue lysates or cell culture supernatants. Researchers should empirically determine the suitability of the TIMP-1 timp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TIMP-1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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