Rat Syndecan 4 (SDC4) ELISA Kit | SDC4 elisa kit

Rat Syndecan 4 (SDC4) ELISA Kit

Cat. Num. AAA450733

• Gene Names: Sdc4; RYUDOCA; RATRYUDOCA
• Reactivity: Rat
• Synonyms: Syndecan 4 (SDC4); Rat Syndecan 4 (SDC4) ELISA Kit; SYND4; Amphiglycan; Ryudocan; Ryudocan core protein; SDC4 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of SDC4. No significant cross-reactivity or interference between SDC4 and analogues was observed.
• Sequence Length: 202

• Samples: Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.133ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SDC4 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SDC4 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SDC4 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of SDC4 in rat serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SDC4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to SDC4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SDC4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of SDC4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6981522
• NCBI GeneID: 24771
• NCBI Accession #: NP_036781.1
• NCBI GenBank Nucleotide #: NM_012649.2
• UniProt Accession #: P34901
• Molecular Weight: 21,962 Da
• NCBI Official Full Name: syndecan-4
• NCBI Official Synonym Full Names: syndecan 4
• NCBI Official Symbol: Sdc4
• NCBI Official Synonym Symbols: RYUDOCA; RATRYUDOCA
• NCBI Protein Information: syndecan-4
• UniProt Protein Name: Syndecan-4
• Protein Family: Syndecan
• UniProt Gene Name: Sdc4
• UniProt Synonym Gene Names: Synd4; SYND4

NCBI Description

regulates cell-matrix interactions; may bind phospholipids and play a role in phosphoinositide-mediated signaling [RGD, Feb 2006]

Uniprot Description

Cell surface proteoglycan that bears heparan sulfate. Regulates exosome biogenesis in concert with SDCBP and PDCD6IP.