Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Taste receptor type 1 member 2 (TAS1R2) Standard Curve.)
Rat Taste Receptor Type 1 Member 2 (TAS1R2) ELISA Kit | TAS1R2 elisa kit
Rat Taste Receptor Type 1 Member 2 (TAS1R2) ELISA Kit
Gene Names
Tas1r2; Tr2; Gpr71; Aldh4a1
Reactivity
Rat
Synonyms
Taste Receptor Type 1 Member 2 (TAS1R2); Rat Taste Receptor Type 1 Member 2 (TAS1R2) ELISA Kit; TAS1R2; GPR71; T1R2; TR2; G protein-coupled receptor 71; TAS1R2 elisa kit
Reactivity
Rat
Specificity
Rat Taste receptor type 1 member 2 (TAS1R2) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TAS1R2. No significant cross-reactivity or interference between Rat TAS1R2 and analogues was observed.
Sequence Length
843
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Taste receptor type 1 member 2 (TAS1R2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Taste receptor type 1 member 2 (TAS1R2) Standard Curve.)
Related Product Information for TAS1R2 elisa kit
Description: This Rat Taste receptor type 1 member 2 (TAS1R2) ELISA Kit employs a two-site sandwich ELISA to quantitate TAS1R2 in samples. An antibody specific for TAS1R2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TAS1R2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TAS1R2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TAS1R2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Taste receptor type 1 member 2 is a protein that in humans is encoded by the TAS1R2 gene.The protein encoded by the TAS1R2 gene is a G protein-coupled receptor with seven trans-membrane domains and is a component of the heterodimeric amino acid taste receptor T1R2+3. This receptor is formed as a dimer of the TAS1R2 and TAS1R3 proteins. Moreover, the TAS1R2 protein is not functional without formation of the 2+3 heterodimer.Another interesting quality of the TAS1R2 and TAS1R1 genes is their spontaneous activity in the absence of the extracellular domains and binding ligands.This may mean that the extracellular domain regulates function of the receptor by preventing spontaneous action as well as binding to activating ligands such as sucrose.
Background: Taste receptor type 1 member 2 is a protein that in humans is encoded by the TAS1R2 gene.The protein encoded by the TAS1R2 gene is a G protein-coupled receptor with seven trans-membrane domains and is a component of the heterodimeric amino acid taste receptor T1R2+3. This receptor is formed as a dimer of the TAS1R2 and TAS1R3 proteins. Moreover, the TAS1R2 protein is not functional without formation of the 2+3 heterodimer.Another interesting quality of the TAS1R2 and TAS1R1 genes is their spontaneous activity in the absence of the extracellular domains and binding ligands.This may mean that the extracellular domain regulates function of the receptor by preventing spontaneous action as well as binding to activating ligands such as sucrose.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
taste receptor type 1 member 2
NCBI Official Symbol
Tas1r2
NCBI Official Synonym Symbols
Tr2; Gpr71; Aldh4a1
NCBI Protein Information
taste receptor type 1 member 2
UniProt Protein Name
Taste receptor type 1 member 2
Protein Family
UniProt Gene Name
Tas1r2
UniProt Synonym Gene Names
Gpr71; T1r2; Tr2
Uniprot Description
Putative taste receptor. TAS1R2/TAS1R3 recognizes diverse natural and synthetic sweeteners.
Research Articles on TAS1R2
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Product Notes
The Rat TAS1R2 tas1r2 (Catalog #AAA9711806) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711806 ELISA Kit recognizes Rat TAS1R2. It is sometimes possible for the material contained within the vial of "Taste Receptor Type 1 Member 2 (TAS1R2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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