Rat Synaptotagmin-1 (SYT1) ELISA Kit | SYT1 elisa kit

Rat Synaptotagmin-1 (SYT1) ELISA Kit

Cat. Num. AAA2801617

• Gene Names: Syt1; P65
• Reactivity: Rat
• Synonyms: Synaptotagmin-1 (SYT1); Rat Synaptotagmin-1 (SYT1) ELISA Kit; DKFZp781D2042; P65; SVP65; SYT; synaptotagmin 1; SYT1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rat SYT1. No significant cross-reactivity or interference between Rat SYT1 and analogues was observed.

• Asssay Type: Quantitive Sandwich
• Sample Types: Serum, Plasma, Other biological fluids
• Detection Wavelength: 450nm
• Sample Volume: 1-200uL
• Precision: Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV<8%; Inter-Assay: CV<12%
• Test Principle: This assay employs a two-site sandwich ELISA to quantitate SYT1 in samples. An antibody specific for SYT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySYT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
• Assay Duration: 1-4.5h
• Preparation and Storage: Store at 2-8 degree C

NCBI and Uniprot Product Information

• NCBI GI #: 148356226
• NCBI GeneID: 25716
• NCBI Accession #: NP_001028852.2
• NCBI GenBank Nucleotide #: NM_001033680.2
• UniProt Accession #: P21707; Q3S2E6; Q707P5
• Molecular Weight: 47,399 Da
• NCBI Official Full Name: synaptotagmin-1
• NCBI Official Synonym Full Names: synaptotagmin I
• NCBI Official Symbol: Syt1
• NCBI Official Synonym Symbols: P65
• NCBI Protein Information: synaptotagmin-1; synaptotagmin 1; sytI
• UniProt Protein Name: Synaptotagmin-1
• Protein Family: Synaptotagmin
• UniProt Gene Name: Syt1
• UniProt Synonym Gene Names: SytI
• UniProt Entry Name: SYT1_RAT

NCBI Description

integral component of synaptic vesicle membranes; important for exocytosis and vesicle trafficking to the active zone of synapses; may be the calcium sensor for calcium-dependent exocytosis [RGD, Feb 2006]

Uniprot Description

SYT1: an integral membrane protein of synaptic vesicles thought to serve as a Ca(2+) sensor in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin I participates in triggering neurotransmitter release at the synapse. Binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2.

Protein type: Calcium-binding; Vesicle; Membrane protein, integral

Cellular Component: neuron projection; synaptic vesicle membrane; cell; integral to membrane; excitatory synapse; terminal button; intracellular organelle; secretory granule; presynaptic membrane; SNARE complex; synaptic vesicle; cytoplasm; plasma membrane; cell junction

Molecular Function: protein C-terminus binding; identical protein binding; clathrin binding; calcium-dependent phospholipid binding; transporter activity; calcium ion binding; calcium-dependent protein binding; calmodulin binding; phosphatidylinositol-4,5-bisphosphate binding; SNARE binding; protein binding; syntaxin binding; low-density lipoprotein receptor binding; phosphatidylserine binding; syntaxin-1 binding; phospholipid binding

Biological Process: positive regulation of calcium ion-dependent exocytosis; synaptic vesicle exocytosis; vesicle docking; regulation of synaptic transmission, glutamatergic; neurotransmitter secretion; positive regulation of vesicle fusion; detection of calcium ion; response to calcium ion; regulation of calcium ion-dependent exocytosis; positive regulation of synaptic transmission; calcium ion-dependent exocytosis