Western Blot (WB)
(Western Blot (WB) analysis of CD52 polyclonal antibody at 1:500 dilutionLane1:THP-1 whole cell lysateLane2:Mouse spleen tissue lysateLane3:H9C2 whole cell lysate)
Rabbit CD52 Polyclonal Antibody | anti-CD52 antibody
CD52 Polyclonal Antibody
Gene Names
CD52; HE5; CDW52; EDDM5
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
CD52; Polyclonal Antibody; CD52 Polyclonal Antibody; CAMPATH-1 antigen; CDw52; Cambridge pathology 1 antigen; Epididymal secretory protein E5; Human epididymis-specific protein 5; He5; CDW52; HE5; anti-CD52 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
CD52 polyclonal antibody detects endogenous levels of CD52 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
61
Applicable Applications for anti-CD52 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
A synthetic peptide corresponding to residues in Human CD52.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of CD52 polyclonal antibody at 1:500 dilutionLane1:THP-1 whole cell lysateLane2:Mouse spleen tissue lysateLane3:H9C2 whole cell lysate)
Western Blot (WB)
(Western Blot (WB) analysis of CD52 polyclonal antibody at 1:500 dilutionLane1:THP-1 whole cell lysateLane2:Mouse spleen tissue lysateLane3:H9C2 whole cell lysate)
Related Product Information for anti-CD52 antibody
CD52 is a glycosylphosphatidylinositol (GPI)-linked surface antigen present at high levels on epithelial cells lining the male reproductive tract, thymocytes, lymphocytes, monocytes and macrophages. It is also present at variable levels on lymphoid malignancies. During sperm maturation, spermatozoa passing through the genital tract acquire CD52 that is shed from the epithelial cell lining into seminal plasma. CD52 is detectable on the surface of epididymal sperm and in the ejaculate but not on spermatogenetic cells or testicular spermatozoa. The peptide backbone of CD52, which consists of 12 amino acids, is considered a mere scaffold for posttranslational modifications, such as GPI-anchor and N-glycosylation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
~ 15kDa
NCBI Official Full Name
CAMPATH-1 antigen
NCBI Official Synonym Full Names
CD52 molecule
NCBI Official Symbol
CD52
NCBI Official Synonym Symbols
HE5; CDW52; EDDM5
NCBI Protein Information
CAMPATH-1 antigen
UniProt Protein Name
CAMPATH-1 antigen
Protein Family
UniProt Gene Name
CD52
UniProt Synonym Gene Names
CDW52; HE5; He5
Uniprot Description
May play a role in carrying and orienting carbohydrate, as well as having a more specific role.
Research Articles on CD52
Similar Products
Product Notes
The CD52 cd52 (Catalog #AAA3006334) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CD52 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CD52 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the CD52 cd52 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD52, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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