Rat Suppressors Of Cytokine Signaling 3 (SOCS3) ELISA Kit | SOCS3 elisa kit

Mini Samples Rat Suppressors Of Cytokine Signaling 3 (SOCS3) ELISA Kit

Cat. Num. AAA2097865

• Gene Names: Socs3; Cish3; Ssi-3; Socs-3
• Reactivity: Rat
• Synonyms: Suppressors Of Cytokine Signaling 3 (SOCS3); Mini Samples Rat Suppressors Of Cytokine Signaling 3 (SOCS3) ELISA Kit; ATOD4; CIS3; Cish3; MGC71791; SOCS-3; SSI-3; SSI3; Cytokine-inducible SH2 protein 3; STAT-induced STAT inhibitor 3; SOCS3 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mini Samples Suppressors Of Cytokine Signaling 3 (SOCS3).; No significant cross-reactivity or interference between Mini Samples Suppressors Of Cytokine Signaling 3 (SOCS3) and analogues was observed.
• Sequence Length: 225

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.061ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Suppressors Of Cytokine Signaling 3 (SOCS3) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Suppressors Of Cytokine Signaling 3 (SOCS3) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SOCS3 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Suppressors Of Cytokine Signaling 3 (SOCS3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Suppressors Of Cytokine Signaling 3 (SOCS3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Suppressors Of Cytokine Signaling 3 (SOCS3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mini Samples Suppressors Of Cytokine Signaling 3 (SOCS3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for SOCS3 elisa kit

Signal transduction; Tumor immunity; Immune molecule; Hepatology

NCBI and Uniprot Product Information

• NCBI GI #: 16758336
• NCBI GeneID: 89829
• NCBI Accession #: NP_446017.1
• NCBI GenBank Nucleotide #: NM_053565.1
• UniProt Accession #: O88583; Q9QYV5
• Molecular Weight: 24,808 Da
• NCBI Official Full Name: suppressor of cytokine signaling 3
• NCBI Official Synonym Full Names: suppressor of cytokine signaling 3
• NCBI Official Symbol: Socs3
• NCBI Official Synonym Symbols: Cish3; Ssi-3; Socs-3
• NCBI Protein Information: suppressor of cytokine signaling 3
• UniProt Protein Name: Suppressor of cytokine signaling 3
• Protein Family: Suppressor of cytokine signaling
• UniProt Gene Name: Socs3
• UniProt Synonym Gene Names: Cish3; SOCS-3

NCBI Description

regulates cytokine levels to modulate inflammation [RGD, Feb 2006]

Uniprot Description

SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. Regulates IL-6 signaling in vivo. Probable substrate-recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize IL6ST ().