Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Steryl-sulfatase (STS) Standard Curve.)
Rat Steryl-Sulfatase (STS) ELISA Kit | STS elisa kit
Rat Steryl-Sulfatase (STS) ELISA Kit
Reactivity
Rat
Synonyms
Steryl-Sulfatase (STS); Rat Steryl-Sulfatase (STS) ELISA Kit; STS; ARSC; ARSC1; ASC; ES; SSDD; XLI; arylsulfatase C; estrone sulfatase; steryl-sulfatase; steryl-sulfate sulfohydrolase; STS elisa kit
Reactivity
Rat
Specificity
Rat Steryl-sulfatase (STS) ELISA Kit has high sensitivity and excellent specificity for detection of Rat STS. No significant cross-reactivity or interference between Rat STS and analogues was observed.
Sequence Length
577
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Steryl-sulfatase (STS) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Steryl-sulfatase (STS) Standard Curve.)
Related Product Information for STS elisa kit
Description: This Rat Steryl-sulfatase (STS) ELISA Kit employs a two-site sandwich ELISA to quantitate STS in samples. An antibody specific for STS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any STS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for STS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of STS bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: STS catalyzes the conversion of sulfated steroid precursors to estrogens during pregnancy. The encoded protein is found in the endoplasmic reticulum, where it acts as a homodimer. The deduced 583-residue protein has a molecular mass of 63 kD and contains a 21- or 23-residue signal peptide, 4 possible N-linked glycosylation sites, and 2 potential membrane-spanning domains. Expression in hamster kidney cells (BHK-21) showed localization predominantly in the endoplasmic reticulum, with smaller fractions found in the Golgi, at the cell surface, and in endosomes and lysosomes. The major transcription start site was at position -221 with respect to the AUG translation initiation codon, and the amino acids were renumbered to make the initiating methionine +1.
Background: STS catalyzes the conversion of sulfated steroid precursors to estrogens during pregnancy. The encoded protein is found in the endoplasmic reticulum, where it acts as a homodimer. The deduced 583-residue protein has a molecular mass of 63 kD and contains a 21- or 23-residue signal peptide, 4 possible N-linked glycosylation sites, and 2 potential membrane-spanning domains. Expression in hamster kidney cells (BHK-21) showed localization predominantly in the endoplasmic reticulum, with smaller fractions found in the Golgi, at the cell surface, and in endosomes and lysosomes. The major transcription start site was at position -221 with respect to the AUG translation initiation codon, and the amino acids were renumbered to make the initiating methionine +1.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
steryl-sulfatase
NCBI Official Synonym Full Names
steroid sulfatase
NCBI Official Symbol
Sts
NCBI Protein Information
steryl-sulfatase
UniProt Protein Name
Steryl-sulfatase
Protein Family
UniProt Gene Name
Sts
UniProt Synonym Gene Names
ASC
UniProt Entry Name
STS_RAT
NCBI Description
enzyme that converts sulfated precursors to estrogen during pregnancy [RGD, Feb 2006]
Uniprot Description
Conversion of sulfated steroid precursors to estrogens during pregnancy.
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Product Notes
The Rat STS sts (Catalog #AAA9711752) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711752 ELISA Kit recognizes Rat STS. It is sometimes possible for the material contained within the vial of "Steryl-Sulfatase (STS), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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