Rat Serine/Threonine-Protein Kinase DCLK1 (DCLK1) ELISA Kit | DCLK1 elisa kit

Rat Serine/Threonine-Protein Kinase DCLK1 (DCLK1) ELISA Kit

Cat. Num. AAA9712464

• Gene Names: Dclk1; Ania4; Cpg16; Dcamkl1
• Reactivity: Rat
• Synonyms: Serine/Threonine-Protein Kinase DCLK1 (DCLK1); Rat Serine/Threonine-Protein Kinase DCLK1 (DCLK1) ELISA Kit; DCLK1; CL1; CLICK1; DCAMKL1; DCDC3A; DCLK; KIAA0369; doublecortin and CaM kinase-like 1; DCLK1 elisa kit

• Reactivity: Rat
• Specificity: Rat Serine/threonine-protein kinase DCLK1 (DCLK1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat DCLK1. No significant cross-reactivity or interference between Rat DCLK1 and analogues was observed.
• Sequence Length: 433

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Rat Serine/threonine-protein kinase DCLK1 (DCLK1) Standard Curve.)

Related Product Information for DCLK1 elisa kit

Description: This Rat Serine/threonine-protein kinase DCLK1 (DCLK1) ELISA Kit employs a two-site sandwich ELISA to quantitate DCLK1 in samples. An antibody specific for DCLK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DCLK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DCLK1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DCLK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Dclk1 encodes a member of the protein kinase superfamily and the doublecortin family. The typical protein encoded by Dclk1 contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the protein is independent of its protein kinase activity. Dclk1 is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis. Multiple transcript variants generated by two alternative promoter usage and alternative splicing have been found, but the full-length nature of the variant produced from the 5' promoter has not been determined. Current reference sequence data represents two alternatively spliced transcript variants produced from the 3' promoter and their protein products lack the doublecortin domain.

NCBI and Uniprot Product Information

• NCBI GI #: 17985955
• NCBI GeneID: 83825
• NCBI Accession #: NP_445795.1
• NCBI GenBank Nucleotide #: NP_445795.1
• UniProt Accession #: O08875
• NCBI Official Full Name: serine/threonine-protein kinase DCLK1 isoform 1
• NCBI Official Synonym Full Names: doublecortin-like kinase 1
• NCBI Official Symbol: Dclk1
• NCBI Official Synonym Symbols: Ania4; Cpg16; Dcamkl1
• NCBI Protein Information: serine/threonine-protein kinase DCLK1
• UniProt Protein Name: Serine/threonine-protein kinase DCLK1
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: Dclk1
• UniProt Synonym Gene Names: Cpg16; Dcamkl1
• UniProt Entry Name: DCLK1_RAT

NCBI Description

This gene encodes a member of the protein kinase superfamily and the doublecortin family. The typical protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the protein is independent of its protein kinase activity. This gene is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis. Multiple transcript variants generated by two alternative promoter usage and alternative splicing have been found, but the full-length nature of the variant produced from the 5' promoter has not been determined. Current reference sequence data represents two alternatively spliced transcript variants produced from the 3' promoter and their protein products lack the doublecortin domain.[provided by RefSeq, Sep 2010]