Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Secreted frizzled-related protein 1 (SFRP1) Standard Curve.)
Rat Secreted Frizzled-Related Protein 1 (SFRP1) ELISA Kit | SFRP1 elisa kit
Rat Secreted Frizzled-Related Protein 1 (SFRP1) ELISA Kit
Gene Names
Sfrp1; sFRP-1
Reactivity
Rat
Synonyms
Secreted Frizzled-Related Protein 1 (SFRP1); Rat Secreted Frizzled-Related Protein 1 (SFRP1) ELISA Kit; SFRP1; FRP; FRP-1; FRP1; FrzA; SARP2; secreted apoptosis-related protein 2; SFRP1 elisa kit
Reactivity
Rat
Specificity
Rat Secreted frizzled-related protein 1 (SFRP1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat SFRP1. No significant cross-reactivity or interference between Rat SFRP1 and analogues was observed.
Sequence Length
158
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Secreted frizzled-related protein 1 (SFRP1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Secreted frizzled-related protein 1 (SFRP1) Standard Curve.)
Related Product Information for SFRP1 elisa kit
Description: This Rat Secreted frizzled-related protein 1 (SFRP1) ELISA Kit employs a two-site sandwich ELISA to quantitate SFRP1 in samples. An antibody specific for SFRP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SFRP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SFRP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SFRP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Secreted frizzled-related protein 1 (SFRP1) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP1 and SFRP5 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP1 is expressed in several human tissues, with the highest levels in heart. SFRP1 is a 35 kDa prototypical member of the SFRP family. It acts as a biphasic modulator of Wnt signaling, counteracting Wnt-induced effects at high concentrations and promoting them at lower concentrations. It is located in a chromosomal region (8p12-p11.1) that is frequently deleted in breast cancer and is thought to harbour a tumor suppressor gene.
Background: Secreted frizzled-related protein 1 (SFRP1) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP1 and SFRP5 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP1 is expressed in several human tissues, with the highest levels in heart. SFRP1 is a 35 kDa prototypical member of the SFRP family. It acts as a biphasic modulator of Wnt signaling, counteracting Wnt-induced effects at high concentrations and promoting them at lower concentrations. It is located in a chromosomal region (8p12-p11.1) that is frequently deleted in breast cancer and is thought to harbour a tumor suppressor gene.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Secreted frizzled-related protein 1
NCBI Official Synonym Full Names
secreted frizzled-related protein 1
NCBI Official Symbol
Sfrp1
NCBI Official Synonym Symbols
sFRP-1
NCBI Protein Information
secreted frizzled-related protein 1
UniProt Protein Name
Secreted frizzled-related protein 1
Protein Family
UniProt Gene Name
Sfrp1
UniProt Synonym Gene Names
sFRP-1
NCBI Description
a secreted protein involved in binding Wnt and antagonizing Wnt signaling [RGD, Feb 2006]
Uniprot Description
Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels (). Has antiproliferative effects on vascular cells, in vitro and in vivo, and can induce, in vivo, an angiogenic response. In vascular cell cycle, delays the G1 phase and entry into the S phase. In kidney development, inhibits tubule formation and bud growth in metanephroi. Inhibits WNT1/WNT4-mediated TCF-dependent transcription.
Research Articles on SFRP1
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Product Notes
The Rat SFRP1 sfrp1 (Catalog #AAA9711889) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711889 ELISA Kit recognizes Rat SFRP1. It is sometimes possible for the material contained within the vial of "Secreted Frizzled-Related Protein 1 (SFRP1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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