Rabbit anti-Human SFRP1 Polyclonal Antibody | anti-SFRP1 antibody

Anti-SFRP1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8103509

• Gene Names: SFRP1; FRP; FRP1; FrzA; FRP-1; SARP2
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: SFRP1; Polyclonal Antibody; Anti-SFRP1 Antibody; Rabbit Polyclonal; SFRP1 Antibody; Rabbit PAb; Antigen Affinity Purified; secreted frizzled-related protein 1; Anti-FRP Antibody; Anti-FRP-1 Antibody; Anti-FRP1 Antibody; Anti-FrzA Antibody; Anti-SARP2 Antibody; anti-SFRP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human SFRP1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-SFRP1 antibody: ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: ELISA: 1:5000-1:10,000; IHC-P: 1:200-1:1000
• Immunogen: Recombinant Human SFRP1 Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human SFRP1 (rh SFRP1). SFRP1 specific IgG was purified by Human SFRP1 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human FRP1 in human kidney with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)

Related Product Information for anti-SFRP1 antibody

Secreted frizzled-related protein 1, also known as sFRP1, is a 35 kDa prototypical member of the SFRP family. SFRP family consists of five secreted glycoproteins in humans acting as extracellular signaling ligands. Each is approximately 3 amino acids in length and contains a cysteine-rich domain (CRD) that shares 3-5% sequence homology with the CRD of Frizzled (Fz) receptors, a putative signal sequence, and a conserved hydrophilic carboxy-terminal domain. SFRPs act as soluble modulators of Wnt signaling, counteracting Wnt-induced effects at high concentrations and promoting them at lower concentrations. SFRPs are able to bind Wnt proteins and Fz receptors in the extracellular compartment. The interaction between SFRPs and Wnt proteins prevents the latter from binding the Fz receptors. The Wnt pathway plays a key role in embryonic development, cell differentiation and cell proliferation. The deregulation of this critical developmental pathway occurs in several human tumor entities. Mouse sFRP1 is highly expressed in kidney and embryonic heart, as well as in the eye, where it is principally localized to the ciliary body and the lens epithelium.

References

Finch P.W., et al., (1997), Purification and molecular cloning of a secreted, Frizzled-related antagonist of Wnt action. Proc. Natl. Acad. Sci. U.S.A. 94:6770-6775.Melkonyan H.S., et al., (1997), SARPs: a family of secreted apoptosis-related proteins.Proc. Natl. Acad. Sci. U.S.A. 94:13636-13641.Zhou Z., et al., (1998), Up-regulation of human secreted frizzled homolog in apoptosis and its down-regulation in breast tumors.Int. J. Cancer 78:95-99.

NCBI and Uniprot Product Information

• NCBI GI #: 56117838
• NCBI GeneID: 6422
• NCBI Accession #: NP_003003.3
• NCBI GenBank Nucleotide #: NM_003012.4
• UniProt Accession #: Q8N474; O00546; O14779
• Molecular Weight: 35,386 Da
• NCBI Official Full Name: secreted frizzled-related protein 1
• NCBI Official Synonym Full Names: secreted frizzled-related protein 1
• NCBI Official Symbol: SFRP1
• NCBI Official Synonym Symbols: FRP; FRP1; FrzA; FRP-1; SARP2
• NCBI Protein Information: secreted frizzled-related protein 1; SARP-2; sFRP-1; secreted apoptosis-related protein 2
• UniProt Protein Name: Secreted frizzled-related protein 1
• Protein Family: Secreted frizzled-related protein
• UniProt Gene Name: SFRP1
• UniProt Synonym Gene Names: FRP; FRP1; SARP2; FRP-1; sFRP-1; SARP-2
• UniProt Entry Name: SFRP1_HUMAN

NCBI Description

This gene encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. Members of this family act as soluble modulators of Wnt signaling; epigenetic silencing of SFRP genes leads to deregulated activation of the Wnt-pathway which is associated with cancer. This gene may also be involved in determining the polarity of photoreceptor cells in the retina. [provided by RefSeq, Sep 2009]

Uniprot Description

SFRP1: Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels. Has antiproliferative effects on vascular cells, in vitro and in vivo, and can induce, in vivo, an angiogenic response. In vascular cell cycle, delays the G1 phase and entry into the S phase. In kidney development, inhibits tubule formation and bud growth in metanephroi. Inhibits WNT1/WNT4-mediated TCF-dependent transcription. Interacts with WNT1, WNT2 and FRZD6. Interacts with WNT4 and WNT8. Down-regulated in colorectal and breast tumors. Up- regulated in uterine leiomyomas under high estrogenic conditions. Expression, in leiomyomal cells, also increased both under hypoxic and serum deprivation conditions. Widely expressed. Absent from lung, liver and peripheral blood leukocytes. Highest levels in heart and fetal kidney. Also expressed in testis, ovary, fetal brain and lung, leiomyomal cells, myometrial cells and vascular smooth muscle cells. Expressed in foreskin fibroblasts and in keratinocytes. Belongs to the secreted frizzled-related protein (sFRP) family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8p11.21

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; cell surface; integral to membrane; extracellular region; plasma membrane; intracellular; cytosol

Molecular Function: heparin binding; G-protein coupled receptor activity; identical protein binding; Wnt-protein binding; Wnt receptor activity; protein binding; frizzled binding; cysteine-type endopeptidase activity; drug binding

Biological Process: negative regulation of osteoclast differentiation; negative regulation of Wnt receptor signaling pathway; somatic stem cell maintenance; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; dorsal/ventral axis specification; Wnt receptor signaling pathway through beta-catenin; proteolysis; response to organic cyclic substance; negative regulation of insulin secretion; negative regulation of BMP signaling pathway; negative regulation of cell proliferation; positive regulation of focal adhesion formation; ureteric bud development; positive regulation of cell proliferation; positive regulation of stress fiber formation; negative regulation of ossification; positive regulation of smoothened signaling pathway; negative regulation of osteoblast differentiation; positive regulation of cell-matrix adhesion; negative regulation of cell migration; positive regulation of Wnt receptor signaling pathway; female gonad development; negative regulation of epithelial cell proliferation; response to drug; negative regulation of B cell differentiation; negative regulation of JNK activity; negative regulation of peptidyl-tyrosine phosphorylation; menstrual cycle phase; male gonad development; digestive tract morphogenesis; cellular response to starvation; positive regulation of cell growth; regulation of angiogenesis; osteoblast differentiation; negative regulation of fibroblast proliferation; neural tube closure; negative regulation of bone remodeling; positive regulation of fat cell differentiation; negative regulation of osteoblast proliferation; hemopoietic progenitor cell differentiation; neural crest cell fate commitment; negative regulation of cell growth; negative regulation of transcription, DNA-dependent; positive regulation of epithelial cell proliferation; negative regulation of apoptosis