Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Reactive oxygen species modulator 1 (ROMO1) Standard Curve.)
Rat Reactive Oxygen Species Modulator 1 (ROMO1) ELISA Kit | ROMO1 elisa kit
Rat Reactive Oxygen Species Modulator 1 (ROMO1) ELISA Kit
Reactivity
Rat
Synonyms
Reactive Oxygen Species Modulator 1 (ROMO1); Rat Reactive Oxygen Species Modulator 1 (ROMO1) ELISA Kit; ROMO1; RP11-353C18.54; C20orf52; MGC111180; MTGMP; bA353C18.2; OTTHUMP00000062052; OTTHUMP00000196684; mitochondrial targeting GXXXG protein; ROMO1 elisa kit
Reactivity
Rat
Specificity
Rat Reactive oxygen species modulator 1 (ROMO1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat ROMO1. No significant cross-reactivity or interference between Rat ROMO1 and analogues was observed.
Sequence Length
79
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Reactive oxygen species modulator 1 (ROMO1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Reactive oxygen species modulator 1 (ROMO1) Standard Curve.)
Related Product Information for ROMO1 elisa kit
Description: This Rat Reactive oxygen species modulator 1 (ROMO1) ELISA Kit employs a two-site sandwich ELISA to quantitate ROMO1 in samples. An antibody specific for ROMO1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ROMO1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ROMO1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ROMO1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The main source of ROS is known to be the mitochondria, and increased levels of ROS from the mitochondria have been observed in many cancer cells. Thus far, the mechanism of ROS production in cancer cell proliferation in the mitochondria is not well-understood. Romo1 increased expression of Romo1-triggered ROS production in the mitochondria. The experiments conducted in the present study showed that Romo1-derived ROS were indispensable for the proliferation of both normal and cancer cells. Furthermore, whilst cell growth was inhibited by blocking the ERK pathway in cells transfected with siRNA directed against Romo1, the cell growth was recovered by addition of exogenous hydrogen peroxide. The results of this study suggest that Romo1-induced ROS may play an important role in redox signaling in cancer cells.
Background: The main source of ROS is known to be the mitochondria, and increased levels of ROS from the mitochondria have been observed in many cancer cells. Thus far, the mechanism of ROS production in cancer cell proliferation in the mitochondria is not well-understood. Romo1 increased expression of Romo1-triggered ROS production in the mitochondria. The experiments conducted in the present study showed that Romo1-derived ROS were indispensable for the proliferation of both normal and cancer cells. Furthermore, whilst cell growth was inhibited by blocking the ERK pathway in cells transfected with siRNA directed against Romo1, the cell growth was recovered by addition of exogenous hydrogen peroxide. The results of this study suggest that Romo1-induced ROS may play an important role in redox signaling in cancer cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
reactive oxygen species modulator 1
UniProt Protein Name
Reactive oxygen species modulator 1
Protein Family
UniProt Gene Name
ROMO1
UniProt Synonym Gene Names
C20orf52; ROS modulator 1; MTGM
UniProt Entry Name
ROMO1_HUMAN
Uniprot Description
ROMO1: Induces production of reactive oxygen species (ROS) which are necessary for cell proliferation. May play a role in inducing oxidative DNA damage and replicative senescence. May play a role in the coordination of mitochondrial morphology and cell proliferation. Belongs to the MGR2 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell cycle regulation; Membrane protein, integral; Mitochondrial
Chromosomal Location of Human Ortholog: 20q11.22
Cellular Component: mitochondrion; mitochondrial inner membrane; integral to membrane
Biological Process: positive regulation of cell proliferation; defense response to bacterium; replicative cell aging
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Product Notes
The Rat ROMO1 romo1 (Catalog #AAA9712669) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712669 ELISA Kit recognizes Rat ROMO1. It is sometimes possible for the material contained within the vial of "Reactive Oxygen Species Modulator 1 (ROMO1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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