Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial (PDHA1) Standard Curve.)
Rat Pyruvate Dehydrogenase E1 Component Subunit alpha, Somatic Form, Mitochondrial (PDHA1) ELISA Kit | PDHA1 elisa kit
Rat Pyruvate Dehydrogenase E1 Component Subunit alpha, Somatic Form, Mitochondrial (PDHA1) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial (PDHA1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial (PDHA1) Standard Curve.)
Background: The pyruvate dehydrogenase complex is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1); dihydrolipoyl transacetylase (DLAT) (E2); and dihydrolipoyl dehydrogenase (DLD) (E3). The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex.Pyruvate dehydrogenase (E1) performs the first two reactions within the pyruvate dehydrogenase complex (PDC): a decarboxylation of substrate 1 (pyruvate) and a reductive acetylation of substrate 2 (lipoic acid).
NCBI and Uniprot Product Information
NCBI Description
catalyzes the interconversion of pyruvate and lipoamide to S-acetyldihydrolipoamide and CO2 [RGD, Feb 2006]
Uniprot Description
PDHA1: a mitochondrial matrix enzyme that catalyzes the oxidative decarboxylation of pyruvate, producing acetyl-CoA and CO2. A key enzyme in controlling the balance between lipid and glucose oxidation depending on substrate availability. The pyruvate dehydrogenase (PDH) holoenzyme is a multi-enzyme complex (PDHC) that contains 20-30 copies of pyruvate decarboxylase tetramers (2 alpha:2 beta)(E1), 60 copies of dihydrolipoamide acetyltransferase (E2), six homodimers of dihydrolipoamide dehydrogenase (E3), plus E3 binding proteins. The activity of PDH is tightly regulated by phosphorylation. The phosphorylation of at least one of three specific serine residues in E1 subunit by PDHK inactivates the PDHC, while dephosphorylation by PDP restores its activity. Sites 1, 2, and 3 of PDHA1 are S293, S300, and S232, respectively. Four PDHK isoenzymes have been described, each with different site specificity: all four phosphorylate sites 1 and 2 but at different rates; for site 1 PDHK2 >PDHK4 >PDHK1 >PDHK3; for site 2, PDHK3> PDHK4 > PDHK2 > PDHK1. Only PDHK1 phosphorylates site 3. PDHA1 deficiency is the most common enzyme defect in patients with primary lactic acidosis.
Protein type: Mitochondrial; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - butanoate; Carbohydrate Metabolism - citrate (TCA) cycle; Amino Acid Metabolism - valine, leucine and isoleucine biosynthesis; EC 1.2.4.1; Oxidoreductase
Cellular Component: intracellular membrane-bound organelle; mitochondrial pyruvate dehydrogenase complex; mitochondrion; myelin sheath; nucleus; pyruvate dehydrogenase complex
Molecular Function: pyruvate dehydrogenase (acetyl-transferring) activity; pyruvate dehydrogenase activity
Biological Process: acetyl-CoA biosynthetic process from pyruvate; glucose metabolic process; tricarboxylic acid cycle
Research Articles on PDHA1
Similar Products
Product Notes
The Rat PDHA1 pdha1 (Catalog #AAA9712135) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712135 ELISA Kit recognizes Rat PDHA1. It is sometimes possible for the material contained within the vial of "Pyruvate Dehydrogenase E1 Component Subunit alpha, Somatic Form, Mitochondrial (PDHA1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
