Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Pyruvate dehydrogenase E1 component subunit beta, mitochondrial (PDHB) Standard Curve.)
Mouse Pyruvate Dehydrogenase E1 Component Subunit beta, Mitochondrial (PDHB) ELISA Kit | PDHB elisa kit
Mouse Pyruvate Dehydrogenase E1 Component Subunit beta, Mitochondrial (PDHB) ELISA Kit
Gene Names
Pdhb; C81408; AL024199; 2610103L06Rik
Reactivity
Mouse
Synonyms
Pyruvate Dehydrogenase E1 Component Subunit beta; Mitochondrial (PDHB); Mouse Pyruvate Dehydrogenase E1 Component Subunit beta; Mitochondrial (PDHB) ELISA Kit; PDHB; DKFZp564K0164; PHE1B; Pyruvate dehydrogenase; E1 beta polypeptide; PDHB elisa kit
Reactivity
Mouse
Specificity
Mouse Pyruvate dehydrogenase E1 component subunit beta, mitochondrial (PDHB) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PDHB. No significant cross-reactivity or interference between Mouse PDHB and analogues was observed.
Sequence Length
359
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Pyruvate dehydrogenase E1 component subunit beta, mitochondrial (PDHB) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Pyruvate dehydrogenase E1 component subunit beta, mitochondrial (PDHB) Standard Curve.)
Related Product Information for PDHB elisa kit
Description: This Mouse Pyruvate dehydrogenase E1 component subunit beta, mitochondrial (PDHB) ELISA Kit employs a two-site sandwich ELISA to quantitate PDHB in samples. An antibody specific for PDHB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PDHB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDHB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDHB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Koike et al. (1988) cloned and sequenced cDNAs encoding the alpha and the beta subunits. Theoretically, there may be 2 forms of pyruvate dehydrogenase deficiency, one with mutation in the PDHA gene and one with mutation in the PDHB gene. Ho et al. (1988) isolated a 1.5-kb cDNA clone for the beta subunit of E1 from a human liver gamma-gt11 cDNA library using anti-E1 serum. Using a cDNA probe, Olson et al. (1990) demonstrated that the PHE1B gene is located on 3p13-q23. Koike et al. (1990) described the molecular cloning of the entire human PHE1B gene, its characterization by restriction enzyme analysis, and its complete nucleotide sequence. The gene is composed of 10 exons and 9 introns. All intron-exon splice junctions follow the GT/AG rule. The Alu family was found in introns 2 and 8.
Background: Koike et al. (1988) cloned and sequenced cDNAs encoding the alpha and the beta subunits. Theoretically, there may be 2 forms of pyruvate dehydrogenase deficiency, one with mutation in the PDHA gene and one with mutation in the PDHB gene. Ho et al. (1988) isolated a 1.5-kb cDNA clone for the beta subunit of E1 from a human liver gamma-gt11 cDNA library using anti-E1 serum. Using a cDNA probe, Olson et al. (1990) demonstrated that the PHE1B gene is located on 3p13-q23. Koike et al. (1990) described the molecular cloning of the entire human PHE1B gene, its characterization by restriction enzyme analysis, and its complete nucleotide sequence. The gene is composed of 10 exons and 9 introns. All intron-exon splice junctions follow the GT/AG rule. The Alu family was found in introns 2 and 8.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
pyruvate dehydrogenase E1 component subunit beta, mitochondrial
NCBI Official Synonym Full Names
pyruvate dehydrogenase (lipoamide) beta
NCBI Official Symbol
Pdhb
NCBI Official Synonym Symbols
C81408; AL024199; 2610103L06Rik
NCBI Protein Information
pyruvate dehydrogenase E1 component subunit beta, mitochondrial
UniProt Protein Name
Pyruvate dehydrogenase E1 component subunit beta, mitochondrial
Protein Family
UniProt Gene Name
Pdhb
UniProt Synonym Gene Names
PDHE1-B
Uniprot Description
The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2, and thereby links the glycolytic pathway to the tricarboxylic cycle.
Research Articles on PDHB
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Product Notes
The Mouse PDHB pdhb (Catalog #AAA9717387) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717387 ELISA Kit recognizes Mouse PDHB. It is sometimes possible for the material contained within the vial of "Pyruvate Dehydrogenase E1 Component Subunit beta, Mitochondrial (PDHB), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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