Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Protein Tob1 (TOB1) Standard Curve.)
Rat Protein Tob1 (TOB1) ELISA Kit | TOB1 elisa kit
Rat Protein Tob1 (TOB1) ELISA Kit
Reactivity
Rat
Synonyms
Protein Tob1 (TOB1); Rat Protein Tob1 (TOB1) ELISA Kit; TOB1; APRO6; MGC104792; MGC34446; PIG49; TOB; TROB; TROB1; transducer of erbB-2; TOB1 elisa kit
Reactivity
Rat
Specificity
Rat Protein Tob1 (TOB1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TOB1. No significant cross-reactivity or interference between Rat TOB1 and analogues was observed.
Sequence Length
365
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Protein Tob1 (TOB1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Protein Tob1 (TOB1) Standard Curve.)
Related Product Information for TOB1 elisa kit
Description: This Rat Protein Tob1 (TOB1) ELISA Kit employs a two-site sandwich ELISA to quantitate TOB1 in samples. An antibody specific for TOB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TOB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TOB1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TOB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: TOB2 belongs to the TOB/BTG1 family of antiproliferative proteins, which are involved in the regulation of cell cycle progression.The deduced 344-amino acid TOB2 polypeptide has a calculated molecular mass of 37 kD. It shares 61% overall sequence homology with the TOB1 protein, and both proteins contain a putative nuclear localization signal in the conserved N-terminal domain. Immunofluorescence analysis revealed localization almost exclusively in the cytoplasm. Northern blot analysis of human adult tissues detected ubiquitous expression of a 4.1-kb transcript, with relatively high expression in skeletal muscle, thymus, and ovary. In situ hybridization in several mouse tissues demonstrated intense hybridization in ovary, with characteristic expression in oocytes. Immunoblot analysis revealed a 43-kD protein.
Background: TOB2 belongs to the TOB/BTG1 family of antiproliferative proteins, which are involved in the regulation of cell cycle progression.The deduced 344-amino acid TOB2 polypeptide has a calculated molecular mass of 37 kD. It shares 61% overall sequence homology with the TOB1 protein, and both proteins contain a putative nuclear localization signal in the conserved N-terminal domain. Immunofluorescence analysis revealed localization almost exclusively in the cytoplasm. Northern blot analysis of human adult tissues detected ubiquitous expression of a 4.1-kb transcript, with relatively high expression in skeletal muscle, thymus, and ovary. In situ hybridization in several mouse tissues demonstrated intense hybridization in ovary, with characteristic expression in oocytes. Immunoblot analysis revealed a 43-kD protein.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Protein Tob1
NCBI Official Synonym Full Names
transducer of ErbB-2.1
NCBI Official Symbol
Tob1
NCBI Protein Information
protein Tob1
UniProt Protein Name
Protein Tob1
Protein Family
UniProt Gene Name
Tob1
UniProt Entry Name
TOB1_RAT
NCBI Description
human homolog is an antiproliferative protein involved in the negative regulation of osteoblast and T cell proliferation [RGD, Feb 2006]
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Product Notes
The Rat TOB1 tob1 (Catalog #AAA9711653) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711653 ELISA Kit recognizes Rat TOB1. It is sometimes possible for the material contained within the vial of "Protein Tob1 (TOB1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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