Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Protein Tob2 (TOB2) Standard Curve.)
Mouse Protein Tob2 (TOB2) ELISA Kit | TOB2 elisa kit
Mouse Protein Tob2 (TOB2) ELISA Kit
Gene Names
Tob2; AV071822; mKIAA1663; 2900090N22Rik; 4930545K18Rik
Reactivity
Mouse
Synonyms
Protein Tob2 (TOB2); Mouse Protein Tob2 (TOB2) ELISA Kit; TOB2; TOB4; TOBL; TROB2; TOB2 elisa kit
Reactivity
Mouse
Specificity
Mouse Protein Tob2 (TOB2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TOB2. No significant cross-reactivity or interference between Mouse TOB2 and analogues was observed.
Sequence Length
345
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Protein Tob2 (TOB2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Protein Tob2 (TOB2) Standard Curve.)
Related Product Information for TOB2 elisa kit
Description: This Mouse Protein Tob2 (TOB2) ELISA Kit employs a two-site sandwich ELISA to quantitate TOB2 in samples. An antibody specific for TOB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TOB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TOB2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TOB2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Target of EGR1 protein 1 is a protein encoded by the TOE1 gene.Egr1, an immediate early transcription factor, responds to diverse stimuli and affects gene transcription to accomplish its biological effects. One important effect of Egr1 expression is to decrease the growth and tumorigenic potential of several tumor cell types. To identify important Egr1 target genes, adapted a methodology involving formaldehyde-induced protein-DNA cross-linking, chromatin immunoprecipitation, and multiplex PCR. Using this approach, report the cloning of a new Egr1 target gene that is able to account, at least in part, for the growth inhibitory activity of Egr1. TOE1 belongs to the CAF1 family. inhibits cell growth rate and cell cycle. Induces CDKN1A expression as well as TGF-beta expression. mediates the inhibitory growth effect of EGR1.
Background: Target of EGR1 protein 1 is a protein encoded by the TOE1 gene.Egr1, an immediate early transcription factor, responds to diverse stimuli and affects gene transcription to accomplish its biological effects. One important effect of Egr1 expression is to decrease the growth and tumorigenic potential of several tumor cell types. To identify important Egr1 target genes, adapted a methodology involving formaldehyde-induced protein-DNA cross-linking, chromatin immunoprecipitation, and multiplex PCR. Using this approach, report the cloning of a new Egr1 target gene that is able to account, at least in part, for the growth inhibitory activity of Egr1. TOE1 belongs to the CAF1 family. inhibits cell growth rate and cell cycle. Induces CDKN1A expression as well as TGF-beta expression. mediates the inhibitory growth effect of EGR1.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
protein Tob2
NCBI Official Synonym Full Names
transducer of ERBB2, 2
NCBI Official Symbol
Tob2
NCBI Official Synonym Symbols
AV071822; mKIAA1663; 2900090N22Rik; 4930545K18Rik
NCBI Protein Information
protein Tob2
UniProt Protein Name
Protein Tob2
Protein Family
UniProt Gene Name
Tob2
UniProt Entry Name
TOB2_MOUSE
Research Articles on TOB2
Similar Products
Product Notes
The Mouse TOB2 tob2 (Catalog #AAA9716693) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716693 ELISA Kit recognizes Mouse TOB2. It is sometimes possible for the material contained within the vial of "Protein Tob2 (TOB2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
