Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Protein lyl-1 (LYL1) Standard Curve.)
Rat Protein Lyl-1 (LYL1) ELISA Kit | LYL1 elisa kit
Rat Protein Lyl-1 (LYL1) ELISA Kit
Reactivity
Rat
Synonyms
Protein Lyl-1 (LYL1); Rat Protein Lyl-1 (LYL1) ELISA Kit; LYL1; bHLHa18; LYL1 elisa kit
Reactivity
Rat
Specificity
Rat Protein lyl-1 (LYL1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat LYL1. No significant cross-reactivity or interference between Rat LYL1 and analogues was observed.
Sequence Length
278
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Protein lyl-1 (LYL1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Protein lyl-1 (LYL1) Standard Curve.)
Related Product Information for LYL1 elisa kit
Description: This Rat Protein lyl-1 (LYL1) ELISA Kit employs a two-site sandwich ELISA to quantitate LYL1 in samples. An antibody specific for LYL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LYL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LYL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LYL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: On chromosome 19, the translocation occurred within a previously uncharacterized transcriptional unit for which Cleary et al. (1988) proposed the designation LYL1. An RNA of about 1.5 kb was transcribed from this gene in a wide variety of hematolymphoid cell lines. The t(7;19) resulted in truncation of the LYL1 gene and production of abnormal-sized RNAs, suggesting a role for LYL1 in the pathogenesis of this leukemia. By fluorescence in situ hybridization, Trask et al. (1993) assigned the LYL1 gene to 19p13.2-p13.1. Kuo et al. (1991) mapped the mouse Lyl-1 gene to chromosome 8, thereby defining a new region of homology of synteny with human 19p. The mapping was achieved in an interspecific backcross using a RFLP. The predicted mouse Lyl-1 protein was 78% identical to human LYL1.
Background: On chromosome 19, the translocation occurred within a previously uncharacterized transcriptional unit for which Cleary et al. (1988) proposed the designation LYL1. An RNA of about 1.5 kb was transcribed from this gene in a wide variety of hematolymphoid cell lines. The t(7;19) resulted in truncation of the LYL1 gene and production of abnormal-sized RNAs, suggesting a role for LYL1 in the pathogenesis of this leukemia. By fluorescence in situ hybridization, Trask et al. (1993) assigned the LYL1 gene to 19p13.2-p13.1. Kuo et al. (1991) mapped the mouse Lyl-1 gene to chromosome 8, thereby defining a new region of homology of synteny with human 19p. The mapping was achieved in an interspecific backcross using a RFLP. The predicted mouse Lyl-1 protein was 78% identical to human LYL1.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
protein lyl-1
NCBI Official Synonym Full Names
LYL1, basic helix-loop-helix family member
NCBI Official Symbol
Lyl1
NCBI Protein Information
protein lyl-1
UniProt Protein Name
Protein lyl-1
Protein Family
UniProt Gene Name
Lyl1
UniProt Entry Name
LYL1_RAT
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Product Notes
The Rat LYL1 lyl1 (Catalog #AAA9712566) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712566 ELISA Kit recognizes Rat LYL1. It is sometimes possible for the material contained within the vial of "Protein Lyl-1 (LYL1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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