Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Prolactin-releasing peptide (PRLH) Standard Curve.)
Rat Prolactin-Releasing Peptide (PRLH) ELISA Kit | PRLH elisa kit
Rat Prolactin-Releasing Peptide (PRLH) ELISA Kit
Gene Names
Prlh; Prh; prrp
Reactivity
Rat
Synonyms
Prolactin-Releasing Peptide (PRLH); Rat Prolactin-Releasing Peptide (PRLH) ELISA Kit; PRLH; PRH; PRRP; preproprolactin-releasing peptide; PRLH elisa kit
Reactivity
Rat
Specificity
Rat Prolactin-releasing peptide (PRLH) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PRLH. No significant cross-reactivity or interference between Rat PRLH and analogues was observed.
Sequence Length
83
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Prolactin-releasing peptide (PRLH) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Prolactin-releasing peptide (PRLH) Standard Curve.)
Related Product Information for PRLH elisa kit
Description: This Rat Prolactin-releasing peptide (PRLH) ELISA Kit employs a two-site sandwich ELISA to quantitate PRLH in samples. An antibody specific for PRLH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRLH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRLH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRLH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: PRH mRNA was expressed in all 5 normal pituitary glands examined. In contrast, PRH mRNA was detected in only 5 of 11 human prolactinomas. All 5 prolactinomas expressing PRH were responsive to dopamine agonist treatment, whereas PRH-negative prolactinomas were non- or partially responsive. PRH mRNA was also detected in 6 of 13 GH-secreting tumors and in 5 of 10 clinically nonfunctioning tumors investigated. PRH receptor mRNA was found in all the normal and neoplastic human pituitary samples studied. The authors concluded that the production of PRH and its receptor by normal and neoplastic pituitary tissue raises the question of whether it may regulate prolactin production in an autocrine/paracrine manner in pituitary tissue.
Background: PRH mRNA was expressed in all 5 normal pituitary glands examined. In contrast, PRH mRNA was detected in only 5 of 11 human prolactinomas. All 5 prolactinomas expressing PRH were responsive to dopamine agonist treatment, whereas PRH-negative prolactinomas were non- or partially responsive. PRH mRNA was also detected in 6 of 13 GH-secreting tumors and in 5 of 10 clinically nonfunctioning tumors investigated. PRH receptor mRNA was found in all the normal and neoplastic human pituitary samples studied. The authors concluded that the production of PRH and its receptor by normal and neoplastic pituitary tissue raises the question of whether it may regulate prolactin production in an autocrine/paracrine manner in pituitary tissue.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
prolactin-releasing peptide
NCBI Official Synonym Full Names
prolactin releasing hormone
NCBI Official Symbol
Prlh
NCBI Official Synonym Symbols
Prh; prrp
NCBI Protein Information
prolactin-releasing peptide
UniProt Protein Name
Prolactin-releasing peptide
Protein Family
UniProt Gene Name
Prlh
UniProt Synonym Gene Names
Prh; PrRP
UniProt Entry Name
PRRP_RAT
Research Articles on PRLH
Similar Products
Product Notes
The Rat PRLH prlh (Catalog #AAA9712062) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712062 ELISA Kit recognizes Rat PRLH. It is sometimes possible for the material contained within the vial of "Prolactin-Releasing Peptide (PRLH), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
