Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Prolactin-releasing peptide receptor (PRLHR) Standard Curve.)
Human Prolactin-Releasing Peptide Receptor (PRLHR) ELISA Kit | PRLHR elisa kit
Human Prolactin-Releasing Peptide Receptor (PRLHR) ELISA Kit
Gene Names
PRLHR; GR3; GPR10; PrRPR
Reactivity
Human
Synonyms
Prolactin-Releasing Peptide Receptor (PRLHR); Human Prolactin-Releasing Peptide Receptor (PRLHR) ELISA Kit; PRLHR; GPR10; GR3; MGC126539; MGC126541; PrRPR; G protein-coupled receptor 10; OTTHUMP00000020584; prolactin releasing peptide receptor; prolactin-releasing hormone receptor; PRLHR elisa kit
Reactivity
Human
Specificity
Human Prolactin-releasing peptide receptor (PRLHR) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRLHR. No significant cross-reactivity or interference between Human PRLHR and analogues was observed.
Sequence Length
370
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Prolactin-releasing peptide receptor (PRLHR) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Prolactin-releasing peptide receptor (PRLHR) Standard Curve.)
Related Product Information for PRLHR elisa kit
Description: This Human Prolactin-releasing peptide receptor (PRLHR) ELISA Kit employs a two-site sandwich ELISA to quantitate PRLHR in samples. An antibody specific for PRLHR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRLHR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRLHR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRLHR bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The prolactin-releasing hormone receptor (PRRP) is a G-protein coupled receptor that binds the prolactin releasing hormone.PRLHR is a 7-transmembrane domain receptor for prolactin-releasing hormone that is highly expressed in anterior pituitary By screening a placenta genomic DNA library, followed by 5-prime and 3-prime RACE of pituitary and adenoma cDNA libraries, Ozawa et al. (2002) isolated a full-length cDNA encoding PRLHR, which they called PRRPR. They identified multiple transcription start sites. The predicted 370-amino acid protein contains 7 hydrophobic regions. Northern blot analysis revealed expression of a 2.2-kb transcript in pituitary adenomas.
Background: The prolactin-releasing hormone receptor (PRRP) is a G-protein coupled receptor that binds the prolactin releasing hormone.PRLHR is a 7-transmembrane domain receptor for prolactin-releasing hormone that is highly expressed in anterior pituitary By screening a placenta genomic DNA library, followed by 5-prime and 3-prime RACE of pituitary and adenoma cDNA libraries, Ozawa et al. (2002) isolated a full-length cDNA encoding PRLHR, which they called PRRPR. They identified multiple transcription start sites. The predicted 370-amino acid protein contains 7 hydrophobic regions. Northern blot analysis revealed expression of a 2.2-kb transcript in pituitary adenomas.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
prolactin-releasing peptide receptor
NCBI Official Synonym Full Names
prolactin releasing hormone receptor
NCBI Official Symbol
PRLHR
NCBI Official Synonym Symbols
GR3; GPR10; PrRPR
NCBI Protein Information
prolactin-releasing peptide receptor
UniProt Protein Name
Prolactin-releasing peptide receptor
Protein Family
UniProt Gene Name
PRLHR
UniProt Synonym Gene Names
GPR10; GR3; PrRP receptor; PrRPR
UniProt Entry Name
PRLHR_HUMAN
NCBI Description
PRLHR is a 7-transmembrane domain receptor for prolactin-releasing hormone (PRLH; MIM 602663) that is highly expressed in anterior pituitary (Ozawa et al., 2002 [PubMed 11923475]).[supplied by OMIM, Mar 2008]
Uniprot Description
GPR10: Receptor for prolactin-releasing peptide (PrRP). Implicated in lactation, regulation of food intake and pain-signal processing. Belongs to the G-protein coupled receptor 1 family.
Protein type: GPCR, family 1; Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral
Chromosomal Location of Human Ortholog: 10q26.13
Cellular Component: integral to plasma membrane; plasma membrane
Molecular Function: G-protein coupled receptor activity; protein binding; neuropeptide receptor activity; neuropeptide Y receptor activity
Biological Process: G-protein coupled receptor protein signaling pathway; neuropeptide signaling pathway; hormone metabolic process; feeding behavior; female pregnancy
Research Articles on PRLHR
Similar Products
Product Notes
The Human PRLHR prlhr (Catalog #AAA9716002) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716002 ELISA Kit recognizes Human PRLHR. It is sometimes possible for the material contained within the vial of "Prolactin-Releasing Peptide Receptor (PRLHR), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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