Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Pro-melanin-concentrating hormone (PMCH) Standard Curve.)
Pro-Melanin-Concentrating Hormone (PMCH) ELISA Kit | PMCH elisa kit
Rat Pro-Melanin-Concentrating Hormone (PMCH) ELISA Kit
Reactivity
Rat
Synonyms
Pro-Melanin-Concentrating Hormone (PMCH); Rat Pro-Melanin-Concentrating Hormone (PMCH) ELISA Kit; PMCH; MCH; PMCH elisa kit
Reactivity
Rat
Specificity
Rat Pro-melanin-concentrating hormone (PMCH) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PMCH. No significant cross-reactivity or interference between Rat PMCH and analogues was observed.
Sequence Length
165
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Pro-melanin-concentrating hormone (PMCH) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Pro-melanin-concentrating hormone (PMCH) Standard Curve.)
Related Product Information for PMCH elisa kit
Description: This Rat Pro-melanin-concentrating hormone (PMCH) ELISA Kit employs a two-site sandwich ELISA to quantitate PMCH in samples. An antibody specific for PMCH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PMCH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PMCH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PMCH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The melanin-concentrating hormone (MCH) is a cyclic neuropeptide isolated initially from salmon pituitary gland and later from rat hypothalamus. In mammals, MCH perikarya are confined largely to the lateral hypothalamus and zona incerta area with extensive neuronal projections throughout the brain, including the neurohypophysis. The anatomic distribution suggests a neurotransmitter or neuromodulator role for MCH in a broad array of neuronal functions directed toward the regulation of goal-directed behavior, such as food intake, and general arousal. MCH and 2 other putative neuropeptides, NEI and NGE, are encoded by the same precursor and appear colocalized in nerve cells and in many instances within the projections. The precursor is designated pro-melanin-concentrating hormone (PMCH).
Background: The melanin-concentrating hormone (MCH) is a cyclic neuropeptide isolated initially from salmon pituitary gland and later from rat hypothalamus. In mammals, MCH perikarya are confined largely to the lateral hypothalamus and zona incerta area with extensive neuronal projections throughout the brain, including the neurohypophysis. The anatomic distribution suggests a neurotransmitter or neuromodulator role for MCH in a broad array of neuronal functions directed toward the regulation of goal-directed behavior, such as food intake, and general arousal. MCH and 2 other putative neuropeptides, NEI and NGE, are encoded by the same precursor and appear colocalized in nerve cells and in many instances within the projections. The precursor is designated pro-melanin-concentrating hormone (PMCH).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
pro-MCH
NCBI Official Synonym Full Names
pro-melanin-concentrating hormone
NCBI Official Symbol
Pmch
NCBI Protein Information
pro-MCH
UniProt Protein Name
Pro-MCH
Protein Family
UniProt Gene Name
Pmch
UniProt Synonym Gene Names
Mch; NGE; Neuropeptide G-E; NEI; Neuropeptide E-I; MCH
UniProt Entry Name
MCH_RAT
NCBI Description
cyclic neuropeptide; induces hippocampal synaptic transmission [RGD, Feb 2006]
Research Articles on PMCH
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Product Notes
The Rat PMCH pmch (Catalog #AAA9712105) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712105 ELISA Kit recognizes Rat PMCH. It is sometimes possible for the material contained within the vial of "Pro-Melanin-Concentrating Hormone (PMCH), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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