ELISA (EIA)
(Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Shb (Phospho-Tyr246) Antibody)
Rabbit anti-Human, Mouse Shb Polyclonal Antibody | anti-Shb antibody
Shb (Phospho-Tyr246) Antibody
Gene Names
SHB; bA3J10.2
Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Synonyms
Shb; Polyclonal Antibody; Shb (Phospho-Tyr246) Antibody; anti-Shb antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
Shb (Phospho-Tyr246) antibody detects endogenous levels of Shb only when phosphorylated at Tyr246.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Form/Format
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
297
Applicable Applications for anti-Shb antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
ELISA: 1:10000
ELISA: 1:10000
Target Modification
Phospho
Modification Sites
H:Y246; M:Y240
Immunogen
The antiserum was produced against synthesized phospho-peptide derived from human Shb around the phosphorylation site of Tyr246.
Preparation and Storage
Stable at -20 degree C for at least 1 year.
ELISA (EIA)
(Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Shb (Phospho-Tyr246) Antibody)
ELISA (EIA)
(Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Shb (Phospho-Tyr246) Antibody)
Western Blot (WB)
(Western blot analysis of lysate from 293 cells, using phospho-Serhb (Phospho-Tyr246) antibody. )
Western Blot (WB)
(Western blot analysis of lysate from 293 cells, using phospho-Serhb (Phospho-Tyr246) antibody. )
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
55 kDa
NCBI Official Full Name
SH2 domain-containing adapter protein B
NCBI Official Synonym Full Names
SH2 domain containing adaptor protein B
NCBI Official Symbol
SHB
NCBI Official Synonym Symbols
bA3J10.2
NCBI Protein Information
SH2 domain-containing adapter protein B
UniProt Protein Name
SH2 domain-containing adapter protein B
Protein Family
UniProt Gene Name
SHB
Uniprot Description
Adapter protein which regulates several signal transduction cascades by linking activated receptors to downstream signaling components. May play a role in angiogenesis by regulating FGFR1, VEGFR2 and PDGFR signaling. May also play a role in T-cell antigen receptor/TCR signaling, interleukin-2 signaling, apoptosis and neuronal cells differentiation by mediating basic-FGF and NGF-induced signaling cascades. May also regulate IRS1 and IRS2 signaling in insulin-producing cells.
Research Articles on Shb
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Product Notes
The Shb shb (Catalog #AAA9508546) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Shb (Phospho-Tyr246) Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Shb can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500~1:1000 ELISA: 1:10000. Researchers should empirically determine the suitability of the Shb shb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Shb, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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