Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Plasminogen activator inhibitor 2 (SERPINB2) Standard Curve.)
Rat Plasminogen Activator Inhibitor 2 (SERPINB2) ELISA Kit | SERPINB2 elisa kit
Rat Plasminogen Activator Inhibitor 2 (SERPINB2) ELISA Kit
Gene Names
Serpinb2; Pai2a
Reactivity
Rat
Synonyms
Plasminogen Activator Inhibitor 2 (SERPINB2); Rat Plasminogen Activator Inhibitor 2 (SERPINB2) ELISA Kit; SERPINB2; HsT1201; PAI; PAI-2; PAI2; PLANH2; plasminogen activator inhibitor 2; plasminogen activator inhibitor; type (arginine-serpin); serine (or cysteine) proteinase inhibitor; clade B (ovalbumin); member; SERPINB2 elisa kit
Reactivity
Rat
Specificity
Rat Plasminogen activator inhibitor 2 (SERPINB2) ELISA Kit has high sensitivity and excellent specificity for detection of Rat SERPINB2. No significant cross-reactivity or interference between Rat SERPINB2 and analogues was observed.
Sequence Length
416
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Plasminogen activator inhibitor 2 (SERPINB2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Plasminogen activator inhibitor 2 (SERPINB2) Standard Curve.)
Related Product Information for SERPINB2 elisa kit
Description: This Rat Plasminogen activator inhibitor 2 (SERPINB2) ELISA Kit employs a two-site sandwich ELISA to quantitate SERPINB2 in samples. An antibody specific for SERPINB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SERPINB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SERPINB2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPINB2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Plasminogen activator inhibitor-2 (placental PAI) is a coagulation factor that inactivates tPA and urokinase. It is present in most cells, especially monocytes/macrophages. PAI-2 exists in two forms, a 60-kDa extracellular glycosylated form and a 43-kDa intracellular form. It is present only at detectable quantities in blood during pregnancy, as it is produced by the placenta, and may explain partially the increased rate of thrombosis during pregnancy. The majority of expressed PAI-2 remains unsecreted due to the presence of an inefficient internal signal peptide.the intron-exon arrangement of PAI2 is identical to that of chicken ovalbumin and Y genes and distinct from that of other members of the serpin superfamily.
Background: Plasminogen activator inhibitor-2 (placental PAI) is a coagulation factor that inactivates tPA and urokinase. It is present in most cells, especially monocytes/macrophages. PAI-2 exists in two forms, a 60-kDa extracellular glycosylated form and a 43-kDa intracellular form. It is present only at detectable quantities in blood during pregnancy, as it is produced by the placenta, and may explain partially the increased rate of thrombosis during pregnancy. The majority of expressed PAI-2 remains unsecreted due to the presence of an inefficient internal signal peptide.the intron-exon arrangement of PAI2 is identical to that of chicken ovalbumin and Y genes and distinct from that of other members of the serpin superfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
plasminogen activator inhibitor 2 type A
NCBI Official Synonym Full Names
serpin family B member 2
NCBI Official Symbol
Serpinb2
NCBI Official Synonym Symbols
Pai2a
NCBI Protein Information
plasminogen activator inhibitor 2 type A
UniProt Protein Name
Plasminogen activator inhibitor 2 type A
Protein Family
UniProt Gene Name
Serpinb2
UniProt Synonym Gene Names
Pai2; Planh2; PAI-2; PAI2A
NCBI Description
plays a role in proteolytic degradation of extracellular matrices in tissue remodeling; mediates the entire process of periodontal wound healing [RGD, Feb 2006]
Uniprot Description
Inhibits urokinase-type plasminogen activator.
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Product Notes
The Rat SERPINB2 serpinb2 (Catalog #AAA9711893) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711893 ELISA Kit recognizes Rat SERPINB2. It is sometimes possible for the material contained within the vial of "Plasminogen Activator Inhibitor 2 (SERPINB2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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