Human Neuroplastin (NPTN) ELISA Kit | NPTN elisa kit

Human Neuroplastin (NPTN) ELISA Kit

Cat. Num. AAA2020968

• Gene Names: Nptn; Sdfr1
• Reactivity: Human
• Synonyms: Neuroplastin (NPTN); Human Neuroplastin (NPTN) ELISA Kit; SDR1; GP55; GP65; SDFR1; np55; np65; Stromal Cell Derived Factor Receptor 1; NPTN elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Neuroplastin (NPTN).; No significant cross-reactivity or interference between Neuroplastin (NPTN) and analogues was observed.
• Sequence Length: 393

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.058ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neuroplastin (NPTN) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neuroplastin (NPTN) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NPTN elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuroplastin (NPTN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neuroplastin (NPTN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neuroplastin (NPTN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neuroplastin (NPTN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for NPTN elisa kit

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 9507073
• NCBI GeneID: 56064
• NCBI Accession #: NP_062253.1
• NCBI GenBank Nucleotide #: NM_019380.1
• UniProt Accession #: P97546; P97547; Q6IRE8
• Molecular Weight: 30,836 Da
• NCBI Official Full Name: neuroplastin
• NCBI Official Synonym Full Names: neuroplastin
• NCBI Official Symbol: Nptn
• NCBI Official Synonym Symbols: Sdfr1
• NCBI Protein Information: neuroplastin
• UniProt Protein Name: Neuroplastin
• Protein Family: Neuroplastin
• UniProt Gene Name: Nptn
• UniProt Synonym Gene Names: Sdfr1; Sdr1; gp55/65; SDR-1
• UniProt Entry Name: NPTN_RAT

NCBI Description

synaptic membrane glycoprotein that is a member of the immunoglobulin (Ig) superfamily [RGD, Feb 2006]

Uniprot Description

neuroplastin: Probable homophilic and heterophilic cell adhesion molecule involved in long term potentiation at hippocampal excitatory synapses through activation of p38MAPK. May also regulate neurite outgrowth by activating the FGFR1 signaling pathway. May play a role in synaptic plasticity. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell development/differentiation

Cellular Component: presynaptic membrane; postsynaptic membrane; cell surface; integral to membrane

Molecular Function: transmembrane receptor protein tyrosine kinase activity; cell adhesion molecule binding; ATP binding; type 1 fibroblast growth factor receptor binding

Biological Process: regulation of cell adhesion; elevation of cytosolic calcium ion concentration; peptidyl-tyrosine phosphorylation; positive regulation of fibroblast growth factor receptor signaling pathway; positive regulation of protein amino acid phosphorylation; homophilic cell adhesion; positive regulation of long-term neuronal synaptic plasticity; transmembrane receptor protein tyrosine kinase signaling pathway