Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Placenta growth factor (PLGF) Standard Curve.)
Rat Placenta Growth Factor (PLGF) ELISA Kit | PLGF elisa kit
Rat Placenta Growth Factor (PLGF) ELISA Kit
Gene Names
Pgf; Plgf
Reactivity
Rat
Synonyms
Placenta Growth Factor (PLGF); Rat Placenta Growth Factor (PLGF) ELISA Kit; PGF; D12S1900; PGFL; PLGF; PlGF-2; SHGC-10760; placenta growth factor; placental growth factor; vascular endothelial growth factor-related protein; placental growth factor-like; PLGF elisa kit
Reactivity
Rat
Specificity
Rat Placenta growth factor (PLGF) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PGF. No significant cross-reactivity or interference between Rat PGF and analogues was observed.
Sequence Length
158
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Placenta growth factor (PLGF) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Placenta growth factor (PLGF) Standard Curve.)
Related Product Information for PLGF elisa kit
Description: This Rat Placenta growth factor (PLGF) ELISA Kit employs a two-site sandwich ELISA to quantitate PGF in samples. An antibody specific for PGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PGF is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PGF bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Placental growth factor (PLGF) is a member of the VEGF (vascular endothelial growth factor) sub-family - a key molecule in angiogenesis and vasculogenesis, in particular during embryogenesis. The main source of PLGF during pregnancy is the placental trophoblast. PLGF is also expressed in many other tissues, including the villous trophoblast. PLGF is a dimeric glycoprotein that, like VEGF, can bind with high affinity to the tyrosine kinase receptor vascular endothelial growth factor receptor 1 (VEGFR-1/Flt-1). PLGF/VEGF heterodimers have been isolated and shown to bind also to the VEGFR-2/KDR/Flk-1 receptor the major mediator of VEGF activities.PLGF shares approximately 42% amino acid (aa) sequence identity with VEGF (53% within the PDGF region), and the two proteins share significant structural similarity.
Background: Placental growth factor (PLGF) is a member of the VEGF (vascular endothelial growth factor) sub-family - a key molecule in angiogenesis and vasculogenesis, in particular during embryogenesis. The main source of PLGF during pregnancy is the placental trophoblast. PLGF is also expressed in many other tissues, including the villous trophoblast. PLGF is a dimeric glycoprotein that, like VEGF, can bind with high affinity to the tyrosine kinase receptor vascular endothelial growth factor receptor 1 (VEGFR-1/Flt-1). PLGF/VEGF heterodimers have been isolated and shown to bind also to the VEGFR-2/KDR/Flk-1 receptor the major mediator of VEGF activities.PLGF shares approximately 42% amino acid (aa) sequence identity with VEGF (53% within the PDGF region), and the two proteins share significant structural similarity.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
placenta growth factor
NCBI Official Synonym Full Names
placental growth factor
NCBI Official Symbol
Pgf
NCBI Official Synonym Symbols
Plgf
NCBI Protein Information
placenta growth factor
UniProt Protein Name
Placenta growth factor
Protein Family
UniProt Gene Name
Pgf
UniProt Synonym Gene Names
Plgf; PlGF
NCBI Description
forms a heterodimer with VEGF; may play a role in vascular endothelial cell proliferation [RGD, Feb 2006]
Uniprot Description
Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Also promotes cell tumor growth ().
Research Articles on PLGF
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Product Notes
The Rat PLGF pgf (Catalog #AAA9712120) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712120 ELISA Kit recognizes Rat PLGF. It is sometimes possible for the material contained within the vial of "Placenta Growth Factor (PLGF), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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