Rabbit anti-Human UBA1 Polyclonal Antibody | anti-UBA1 antibody

UBA1, NT (UBA1, A1S9T, UBE1, Ubiquitin-like modifier-activating enzyme 1, Protein A1S9, Ubiquitin-activating enzyme E1) (APC)

Cat. Num. AAA6360520

• Gene Names: UBA1; A1S9; A1ST; GXP1; UBE1; A1S9T; AMCX1; POC20; SMAX2; UBA1A; UBE1X
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: UBA1; Polyclonal Antibody; NT (UBA1; A1S9T; UBE1; Ubiquitin-like modifier-activating enzyme 1; Protein A1S9; Ubiquitin-activating enzyme E1) (APC); Ubiquitin-activating enzyme E1; anti-UBA1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-UBA1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: UBA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human UBA1.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(UBA1 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the UBA1 antibody detected the UBA1 protein (arrow).)

Immunohistochemistry (IHC)

(UBA1 Antibody (N-term) (AP14555a)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UBA1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-UBA1 antibody

The protein encoded by this gene catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternatively spliced transcript variants that encode the same protein have been described.

Product Categories/Family for anti-UBA1 antibody

Antibodies; Ubiquitin

References

Burkhardt, J., et al. J. Rheumatol. 36(10):2149-2157(2009). Su, Z.L., et al. Leuk. Lymphoma 49(9):1821-1822(2008). Ramser, J., et al. Am. J. Hum. Genet. 82(1):188-193(2008). Carbia-Nagashima, A., et al. Cell 131(2):309-323(2007). Jin, J., et al. Nature 447(7148):1135-1138(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 23510340
• NCBI GeneID: 7317
• NCBI Accession #: NP_695012.1; NP_003325.2
• NCBI GenBank Nucleotide #: NM_153280.2
• UniProt Accession #: P22314; Q5JRR8; Q96E13
• Molecular Weight: ~122kD
• NCBI Official Full Name: ubiquitin-like modifier-activating enzyme 1
• NCBI Official Synonym Full Names: ubiquitin-like modifier activating enzyme 1
• NCBI Official Symbol: UBA1
• NCBI Official Synonym Symbols: A1S9; A1ST; GXP1; UBE1; A1S9T; AMCX1; POC20; SMAX2; UBA1A; UBE1X
• NCBI Protein Information: ubiquitin-like modifier-activating enzyme 1; POC20 centriolar protein homolog; UBA1, ubiquitin-activating enzyme E1 homolog A; A1S9T and BN75 temperature sensitivity complementing
• UniProt Protein Name: Ubiquitin-like modifier-activating enzyme 1
• Protein Family: Ubiquitin-activating enzyme
• UniProt Gene Name: UBA1
• UniProt Synonym Gene Names: A1S9T; UBE1
• UniProt Entry Name: UBA1_HUMAN

NCBI Description

The protein encoded by this gene catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternatively spliced transcript variants that encode the same protein have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

UBE1: an enzyme of the ubiquitin-activating E1 family. Adenylates ubiquitin at its carboxy-terminal glycine residue and thereafter links this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thiolester. There are two active sites within the E1 molecule, allowing it to accomodate two ubiquitin moieties at a time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site. Complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair.

Protein type: Ubiquitin ligase; EC 6.3.2.19; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: Xp11.23

Cellular Component: heterochromatin; desmosome; mitochondrion; lysosomal membrane; cytoplasm; endosome membrane; rough endoplasmic reticulum membrane; nucleus; cytosol

Molecular Function: protein binding; ubiquitin-protein ligase activity; ubiquitin activating enzyme activity; ATP binding

Biological Process: modification-dependent protein catabolic process; protein ubiquitination; response to DNA damage stimulus

Disease: Spinal Muscular Atrophy, X-linked 2