Typical Testing Data/Standard Curve (for reference only)
Rat Placenta Growth Factor (PLGF) ELISA Kit | PLGF elisa kit
Rat Placenta Growth Factor (PLGF) ELISA Kit
Gene Names
Pgf; PIGF; Plgf; AI854365
Reactivity
Rat
Synonyms
Placenta Growth Factor (PLGF); Rat Placenta Growth Factor (PLGF) ELISA Kit; PlGF2; PGF; PGFL; Placental Growth Factor-Like; Vascular Endothelial Growth Factor-Related Protein; PLGF elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Placenta Growth Factor (PLGF).
No significant cross-reactivity or interference between Placenta Growth Factor (PLGF) and analogues was observed.
No significant cross-reactivity or interference between Placenta Growth Factor (PLGF) and analogues was observed.
Sequence Length
158
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
Detection Range
7.81-500pg/mL
Sensitivity
< 2.7pg/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Placenta Growth Factor (PLGF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Placenta Growth Factor (PLGF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PLGF elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Placenta Growth Factor (PLGF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Placenta Growth Factor (PLGF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Placenta Growth Factor (PLGF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Placenta Growth Factor (PLGF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for PLGF elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
17,876 Da
NCBI Official Full Name
placenta growth factor isoform 1
NCBI Official Synonym Full Names
placental growth factor
NCBI Official Symbol
Pgf
NCBI Official Synonym Symbols
PIGF; Plgf; AI854365
NCBI Protein Information
placenta growth factor
UniProt Protein Name
Placenta growth factor
Protein Family
UniProt Gene Name
Pgf
UniProt Synonym Gene Names
Plgf; PlGF
UniProt Entry Name
PLGF_MOUSE
Uniprot Description
PGF: Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. Also promotes cell tumor growth. Belongs to the PDGF/VEGF growth factor family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Secreted
Cellular Component: extracellular space; membrane; extracellular region
Molecular Function: protein homodimerization activity; growth factor activity; protein heterodimerization activity
Biological Process: positive regulation of angiogenesis; ureteric bud branching; positive regulation of cell division; multicellular organismal development; positive regulation of cell proliferation; positive regulation of endothelial cell proliferation; sprouting angiogenesis; angiogenesis; cell differentiation
Research Articles on PLGF
1. Fine branching angiogenesis of anti-mesometrial vessels required both conceptus and maternal PGF.
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Product Notes
The Rat PLGF pgf (Catalog #AAA2018961) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2018961 ELISA Kit recognizes Rat PLGF. It is sometimes possible for the material contained within the vial of "Placenta Growth Factor (PLGF), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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