Typical Testing Data/Standard Curve (for reference only)

Rat Dermatan Sulfate (DS) ELISA Kit | DS elisa kit

Rat Dermatan Sulfate (DS) ELISA Kit

Gene Names

DSE; DSEP; DSEPI; SART2; EDSMC2; SART-2

Reactivity

Rat

Synonyms

Dermatan Sulfate (DS); Rat Dermatan Sulfate (DS) ELISA Kit; DS elisa kit

Ordering
Cat. Num. AAA263602 For Research Use Only!

Reactivity

Rat

Specificity

No cross-reaction with other factors.

Sequence Length

958

Samples

Rat serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant DS concentration

Assay Type

Sandwich

Detection Range

5000 pg/ml-78 pg/ml

Sensitivity

20 pg/ml.

Intra-assay Precision

? 8%

Inter-assay Precision

? 12%

Preparation and Storage

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DS elisa kit

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat DS monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

NCBI GI #

7019521

NCBI GeneID

29940

NCBI Accession #

NP_037484.1

NCBI GenBank Nucleotide #

NM_013352.2

Molecular Weight

109,773 Da

NCBI Official Full Name

dermatan-sulfate epimerase

NCBI Official Synonym Full Names

dermatan sulfate epimerase

NCBI Official Symbol

DSE

NCBI Official Synonym Symbols

DSEP; DSEPI; SART2; EDSMC2; SART-2

NCBI Protein Information

dermatan-sulfate epimerase; DS epimerase; chondroitin-glucuronate 5-epimerase; squamous cell carcinoma antigen recognized by T-cells 2

UniProt Protein Name

Dermatan-sulfate epimerase

UniProt Gene Name

DSE

UniProt Synonym Gene Names

SART2; DS epimerase; SART-2

UniProt Entry Name

DSE_HUMAN

NCBI Description

The protein encoded by this gene is a tumor-rejection antigen. This antigen possesses tumor epitopes capable of inducing HLA-A24-restricted and tumor-specific cytotoxic T lymphocytes in cancer patients and may be useful for specific immunotherapy. This gene product is localized to the endoplasmic reticulum. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

SART2: Converts D-glucuronic acid to L-iduronic acid (IdoUA) residues. Belongs to the dermatan-sulfate isomerase family.

Protein type: EC 5.1.3.19; Isomerase; Glycan Metabolism - chondroitin sulfate biosynthesis; Membrane protein, multi-pass; Membrane protein, integral; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 6q22

Cellular Component: Golgi membrane; Golgi apparatus; endoplasmic reticulum; integral to membrane

Molecular Function: chondroitin-glucuronate 5-epimerase activity

Biological Process: chondroitin sulfate metabolic process; chondroitin sulfate biosynthetic process; glycosaminoglycan metabolic process; heparan sulfate proteoglycan biosynthetic process; carbohydrate metabolic process; pathogenesis; dermatan sulfate biosynthetic process

Disease: Ehlers-danlos Syndrome, Musculocontractural Type 2

Research Articles on DS

1. study identified a homozygous DSE missense mutation (c.803C>T, p.S268L) in a male child with musculocontractural type of Ehlers-Danlos syndrome; data indicate mutation affects the epimerase activity, resulting in reduced dermatan sulfate (DS) biosynthesis and an increased synthesis or an accumulation or reduced conversion of chondroitin sulfate

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Product Notes

The Rat DS dse (Catalog #AAA263602) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA263602 ELISA Kit recognizes Rat DS. It is sometimes possible for the material contained within the vial of "Dermatan Sulfate (DS), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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Rat Dermatan Sulfate (DS) ELISA Kit | DS elisa kit