Western Blot (WB)
(Western blot analysis of extracts from HUVEC cells, using ZDHHC7 antibody.)
Rabbit anti-Human ZDHHC7 Polyclonal Antibody | anti-ZDHHC7 antibody
ZDHHC7 Antibody
Gene Names
ZDHHC7; DHHC7; SERZ1; SERZ-B; ZNF370
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
ZDHHC7; Polyclonal Antibody; ZDHHC7 Antibody; DHHC 7; DHHC7; FLJ10792; FLJ20279; Palmitoyltransferase ZDHHC7; ZDHHC 7; Zinc finger DHHC domain containing 7; Zinc finger DHHC domain containing protein 7; Zinc finger DHHC type containing 7; Zinc finger protein 370; ZNF 370; ZNF370; anti-ZDHHC7 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
ZDHHC7 antibody detects endogenous levels of total ZDHHC7
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
345
Applicable Applications for anti-ZDHHC7 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Membrane; Multipass Membrane Protein
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep
Similarity
Pig (91%), Bovine (82%), Horse (82%), Sheep (82%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from HUVEC cells, using ZDHHC7 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from HUVEC cells, using ZDHHC7 antibody.)
Immunofluorescene (IF)
(MBS9605730 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescene (IF)
(MBS9605730 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Related Product Information for anti-ZDHHC7 antibody
Function: Palmitoyltransferase with broad specificity. Palmitoylates SNAP25 and DLG4/PSD95 (By similarity). Palmitoylates sex steroid hormone receptors, including ESR1, PGR and AR, thereby regulating their targeting to the plasma membrane and their function in rapid intracellular signaling upon binding of sex hormones (PubMed:22031296). May play a role in follicle stimulation hormone (FSH) activation of testicular Sertoli cells (By similarity).
Post-translational Modifications: Autopalmitoylated.
Similarity: The DHHC domain is required for palmitoyltransferase activity. Belongs to the DHHC palmitoyltransferase family.
Post-translational Modifications: Autopalmitoylated.
Similarity: The DHHC domain is required for palmitoyltransferase activity. Belongs to the DHHC palmitoyltransferase family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 35 kD
Predicted: 36 kDa
Predicted: 36 kDa
NCBI Official Full Name
palmitoyltransferase ZDHHC7 isoform 1
NCBI Official Synonym Full Names
zinc finger DHHC-type containing 7
NCBI Official Symbol
ZDHHC7
NCBI Official Synonym Symbols
DHHC7; SERZ1; SERZ-B; ZNF370
NCBI Protein Information
palmitoyltransferase ZDHHC7
UniProt Protein Name
Palmitoyltransferase ZDHHC7
Protein Family
UniProt Gene Name
ZDHHC7
UniProt Synonym Gene Names
DHHC-7
Uniprot Description
Palmitoyltransferase with broad specificity (PubMed:22031296, PubMed:28196865). Palmitoylates JAM3 (PubMed:28196865). Palmitoylates SNAP25 and DLG4/PSD95 (). Palmitoylates sex steroid hormone receptors, including ESR1, PGR and AR, thereby regulating their targeting to the plasma membrane and their function in rapid intracellular signaling upon binding of sex hormones (PubMed:22031296). May play a role in follicle stimulation hormone (FSH) activation of testicular Sertoli cells ().
Research Articles on ZDHHC7
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Product Notes
The ZDHHC7 zdhhc7 (Catalog #AAA9605730) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ZDHHC7 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ZDHHC7 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the ZDHHC7 zdhhc7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZDHHC7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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