Rat Paired Box Gene 6 (PAX6) ELISA Kit | PAX6 elisa kit

Rat Paired Box Gene 6 (PAX6) ELISA Kit

Cat. Num. AAA2706396

• Gene Names: PAX6; AN; AN2; FVH1; MGDA; WAGR; ASGD5; D11S812E
• Reactivity: Rat
• Synonyms: Paired Box Gene 6 (PAX6); Rat Paired Box Gene 6 (PAX6) ELISA Kit; AN; AN2; MGDA; WAGR; Oculorhombin; Aniridia; Keratitis; Aniridia type II protein; PAX6 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Paired Box Gene 6 (PAX6).; No significant cross-reactivity or interference between Paired Box Gene 6 (PAX6) and analogues was observed.
• Sequence Length: –

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.057ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Paired Box Gene 6 (PAX6) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Paired Box Gene 6 (PAX6) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PAX6 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Paired Box Gene 6 (PAX6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Paired Box Gene 6 (PAX6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Paired Box Gene 6 (PAX6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Paired Box Gene 6 (PAX6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 119588642
• NCBI GeneID: 5080
• UniProt Accession #: P26367; Q6N006; Q99413
• Molecular Weight: – Da
• NCBI Official Full Name: paired box gene 6 (aniridia, keratitis), isoform CRA_c
• NCBI Official Synonym Full Names: paired box 6
• NCBI Official Symbol: PAX6
• NCBI Official Synonym Symbols: AN; AN2; FVH1; MGDA; WAGR; ASGD5; D11S812E
• NCBI Protein Information: paired box protein Pax-6
• UniProt Protein Name: Paired box protein Pax-6
• Protein Family: Paired box protein
• UniProt Gene Name: PAX6
• UniProt Synonym Gene Names: AN2

NCBI Description

This gene encodes a homeobox and paired domain-containing protein that binds DNA and functions as a regulator of transcription. Activity of this protein is key in the development of neural tissues, particularly the eye. This gene is regulated by multiple enhancers located up to hundreds of kilobases distant from this locus. Mutations in this gene or in the enhancer regions can cause ocular disorders such as aniridia and Peter's anomaly. Use of alternate promoters and alternative splicing result in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2015]

Uniprot Description

Transcription factor with important functions in the development of the eye, nose, central nervous system and pancreas. Required for the differentiation of pancreatic islet alpha cells (). Competes with PAX4 in binding to a common element in the glucagon, insulin and somatostatin promoters. Regulates specification of the ventral neuron subtypes by establishing the correct progenitor domains (). Isoform 5a appears to function as a molecular switch that specifies target genes.