Rat Osteocalcin/Bone Gla Protein (OT/BGP) ELISA Kit | OT/BGP elisa kit

Rat Osteocalcin/Bone Gla Protein (OT/BGP) ELISA Kit

Cat. Num. AAA25677

• Gene Names: BGLAP; OC; BGP; OCN
• Reactivity: Rat
• Synonyms: Osteocalcin/Bone Gla Protein (OT/BGP); Rat Osteocalcin/Bone Gla Protein (OT/BGP) ELISA Kit; OT/BGP elisa kit

• Reactivity: Rat
• Specificity: No significant cross-reactivity or interference between Rat OT/BGP and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Assay Type: Sandwich
• Detection Range: 1.56 ng/ml – 50 ng/ml.
• Sensitivity: 0.1 ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated OT/BGP concentrations in Rat serum, plasma and other body fluids. Using Purified Rat OT/BGP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add OT/BGP and OT/BGP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of OT/BGP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for OT/BGP elisa kit

Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated OT/BGP concentrations in Rat serum, plasma and other body fluids. Using Purified Rat OT/BGP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add OT/BGP and OT/BGP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of OT/BGP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 40316933
• NCBI GeneID: 632
• NCBI Accession #: NP_954642.1
• NCBI GenBank Nucleotide #: NM_199173.4
• UniProt Accession #: P02818; Q5TCK6
• Molecular Weight: 10,963 Da
• NCBI Official Full Name: osteocalcin preproprotein
• NCBI Official Synonym Full Names: bone gamma-carboxyglutamate (gla) protein
• NCBI Official Symbol: BGLAP
• NCBI Official Synonym Symbols: OC; BGP; OCN
• NCBI Protein Information: osteocalcin; bone Gla protein; gamma-carboxyglutamic acid-containing protein; bone gamma-carboxyglutamate (gla) protein (osteocalcin)
• UniProt Protein Name: Osteocalcin
• UniProt Gene Name: BGLAP
• UniProt Synonym Gene Names: BGP
• UniProt Entry Name: OSTCN_HUMAN

NCBI Description

This gene encodes a highly abundant bone protein secreted by osteoblasts that regulates bone remodeling and energy metabolism. The encoded protein contains a Gla (gamma carboxyglutamate) domain, which functions in binding to calcium and hydroxyapatite, the mineral component of bone. Serum osteocalcin levels may be negatively correlated with metabolic syndrome. Read-through transcription exists between this gene and the neighboring upstream gene, PMF1 (polyamine-modulated factor 1), but the encoded protein only shows sequence identity with the upstream gene product. [provided by RefSeq, Jun 2015]

Uniprot Description

osteocalcin: Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. Belongs to the osteocalcin/matrix Gla protein family.

Protein type: Secreted; Secreted, signal peptide; Cell development/differentiation

Chromosomal Location of Human Ortholog: 1q22

Cellular Component: Golgi apparatus; extracellular space; rough endoplasmic reticulum; cytoplasm; dendrite; perikaryon

Molecular Function: structural constituent of bone; hydroxyapatite binding; calcium ion binding; structural molecule activity

Biological Process: response to drug; response to gravity; regulation of bone resorption; response to glucocorticoid stimulus; cell aging; response to testosterone stimulus; bone mineralization; osteoblast development; odontogenesis; osteoblast differentiation; response to vitamin D; regulation of osteoclast differentiation; response to ethanol; response to vitamin K; response to zinc ion; response to mechanical stimulus; response to estrogen stimulus; response to hydroxyisoflavone; response to activity; cell adhesion; regulation of bone mineralization; skeletal development