NOV active protein

Human NOV

Cat. Num. AAA695717

• Gene Names: NOV; CCN3; NOVh; IBP-9; IGFBP9; IGFBP-9
• Reactivity: Species Reactivity: Human; Cross Reactivity: Mouse
• Purity: Above 95% as determined by SDS-PAGE Analysis.
• Synonyms: NOV; Human NOV; Recombinant Human NOV; NOV active protein

• Host: E Coli
• Reactivity: Species Reactivity: Human; Cross Reactivity: Mouse
• Purity/Purification: Above 95% as determined by SDS-PAGE Analysis.
• Sequence: MQVAATQRC PPQCPGRCP ATPPTCAPG VRAVLDGCS CCLVCARQR GESCSDLEP CDESSGLYC DRSADPSNQ TGICTAVEG DNCVFDGVI YRSGEKFQP SCKFQCTCR DGQIGCVPR CQLDVLLPE PNCPAPRKV EVPGECCEK WICGPDEED SLGGLTLAA YRPEATLGV EVSDSSVNC IEQTTEWTA CSKSCGMGF STRVTNRNR QCEMLKQTR LCMVRPCEQ EPEQPTDKK GKKCLRTKK SLKAIHLQF KNCTSLHTY KPRFCGVCS DGRCCTPHN TKTIQAEFQ CSPGQIVKK PVMVIGTCT CHTNCPKNN EAFLQELEL KTTRGKM

• Biological Activity: Determined by a cell proliferation assay using BALB/c 3T3 cells. The expected ED50 for this effect is 1.0-2.0 mug/ml
• Preparation and Storage: At -20 degree C

Testing Data

Related Product Information for NOV active protein

NOV is a member of the CCN family of secreted cysteine rich regulatory proteins. The full length NOV protein contains four structural domains that confer distinct, and sometimes opposing, biological activities. Elevated expression of NOV is associated with certain tumors, including Wilm's tumor and most nephroblastomas. However, in other tumor types and certain cancer cell lines, increased tumorgenicity and proliferation is correlated with decreased NOV expression. Additionally, NOV induces cell adhesion and cell migration by signaling through specific cell surface integrins and by binding to heparin sulfate proteoglycans and to fibulin 1C. NOV has also been reported to exert proangiogenic activities. Recombinant human NOV is a 36.2 kDa protein containing 331 amino acid residues. It is composed of four distinct structural domains (modules); the IGF binding protein (IGFBP) domain, the von Willebrand Factor C (VWFC) domain, the Thrombospondin type-I (TSP type-1) domain, and a C-terminal cysteine knot-like domain (CTCK).

Product Categories/Family for NOV active protein

Cancer; Angiogenesis; Proliferation and Cell Cycle

NCBI and Uniprot Product Information

• NCBI GI #: 1352515
• NCBI GeneID: 4856
• UniProt Accession #: P48745; Q6I9S3; Q96BY5; Q9UDE4; B2R5X7
• Molecular Weight: 39,162 Da
• NCBI Official Full Name: Protein NOV homolog
• NCBI Official Synonym Full Names: nephroblastoma overexpressed
• NCBI Official Symbol: NOV
• NCBI Official Synonym Symbols: CCN3; NOVh; IBP-9; IGFBP9; IGFBP-9
• NCBI Protein Information: protein NOV homolog; CCN family member 3; IGF-binding protein 9; insulin-like growth factor-binding protein 9; nephroblastoma-overexpressed gene protein homolog
• UniProt Protein Name: Protein NOV homolog
• Protein Family: Novel plant
• UniProt Gene Name: NOV
• UniProt Synonym Gene Names: CCN3; IGFBP9; NOVH; NovH; IBP-9; IGF-binding protein 9; IGFBP-9
• UniProt Entry Name: NOV_HUMAN

NCBI Description

The protein encoded by this gene is a small secreted cysteine-rich protein and a member of the CCN family of regulatory proteins. CNN family proteins associate with the extracellular matrix and play an important role in cardiovascular and skeletal development, fibrosis and cancer development. [provided by RefSeq, Feb 2009]

Uniprot Description

NOV: Immediate-early protein likely to play a role in cell growth regulation. Belongs to the CCN family. Expression is down-regulated by WT1. Interacts with FBLN1.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8q24.1

Cellular Component: proteinaceous extracellular matrix; intracellular membrane-bound organelle; cell soma; axon; dendrite

Molecular Function: heparin binding; integrin binding; insulin-like growth factor binding; growth factor activity

Biological Process: cell-cell signaling; regulation of gene expression; regulation of cell growth; signal transduction; cell adhesion